Mehwish Shareef (2009-VA-562)
Identification Of Genetic Marker In Gh, Igf-1 And Bmp15 Candidate Genes Associated With Growth Trait In Beetal Goat - 2017. - 61p.;
Goats are pivotal indigenous assets of Pakistan and have an ample share in the annual production of milk and meat besides providing enough income to rural families. Beetal are famous for the meat and milk production and found throughout the Punjab province. But information regarding their genetic potential for meat production is still insufficientand need comprehensive genetic and genomic research. There is significant need to explore the candidate genes for growth traits in goat.GH, IGF-1 and BMP15 genes have been reported as candidate genes because they have a role in growth and skeletal development. It was hypothesized that polymorphism may be present in GH gene, IGF-1, and BMP15 genes which might also be associated with body weight and body measurements in Beetal goat. In the present study, 5 exons of growth hormone (GH) gene, exon 4 and partial region of intron 4 of IGF-1 gene and exon2 of BMP15 gene were amplified from goat genomic DNA.To assess this assumption, a total of 60 adult healthy animals of Beetal goat were selected on the basis of maximum variation in their body weight and body measurement (Body Length, height and heart girth). Animals were divided into two body weight categories, i.e. heavy and low.After blood collection (5ml), genomic DNA was extracted from the whole blood and stored at -20ºC for further use. DNA quantification was performed through agarose gel electrophoresis with the help of standard. PCR conditions were optimized. The genotyping was done through RFLP. General Linear Model (GLM) procedure of the Statistical Analysis System package (SAS Institute Inc, version 9.1) was used to test the effectof genotype on the body weight, body length, body height, and chest girth.Present study resulted in the identification ofsome SNP in GH, IGF-1 candidate genes in Beetalgoats. Genotyping results were monomorphic in GH, and BMP15 genes in animals of studiedbreed, while IGF-1 gene was polymorphic and had significant associationof genotypes with body weight and with body measurements. Genotype AA is significantly associated with low body weight having 38.18 Kg body weight and heart girth 33.65 cm, (P<0.05). AA genotype is also significantly associated with body leangth (P<0.05). The goats having BB genotype is significantly associated with high body weight having body weight 47.13 Kg (P<0.05). Genotype AB is significantly associated with withers height (31.93 cm).The sequencing results of the IGF-1 gene showed conserved and showed no other mutation in studied exonUponsequencing,polymorphism was found in GH gene inhigher and lower body weight animals. One SNP was identified in exon 2. The Non-synonymous substitution mutation (A > G) of CGA to CAA at position 825 caused an amino acid change from threonine into Alanine. While one deletion mutation in intron 4was identifiedat position 1546 in this gene. Up-to our knowledge, this is the first study of growth trait genes in the Beetal goat breed of Pakistan and helpful to understand the genetics of growth genes in Beetal goat breed. It may lead us to the identification of useful molecular markers for future use in the selection of animals with better growth traits.
CONCLUSION
By evaluating the genotyping and sequencingresults of all the target regions of growth hormone GH, IGF-1 and BMP15 genes, it is concluded that GHgene and BMP15 gene in beetal goat are monomorphic. One substitution mutation in exon 2 G>Aat position 825 along with one deletionin intron 4at position 1546 can be used as genetic markers in selection programme of breeding. WhileIGF-1 is of prime importance because IGF-1 gene was polymorphic and had significant association with body weight and measurements.
RECOMMENDATION
Due to limited resources 60 animals were used to determine polymorphism in three growth genesand their association with growth traits. It is recommended for further studies complete gene could be genotyped and sequenced and more number of animals should be used to evaluate these traits in future.
Genetics
2788-T
Identification Of Genetic Marker In Gh, Igf-1 And Bmp15 Candidate Genes Associated With Growth Trait In Beetal Goat - 2017. - 61p.;
Goats are pivotal indigenous assets of Pakistan and have an ample share in the annual production of milk and meat besides providing enough income to rural families. Beetal are famous for the meat and milk production and found throughout the Punjab province. But information regarding their genetic potential for meat production is still insufficientand need comprehensive genetic and genomic research. There is significant need to explore the candidate genes for growth traits in goat.GH, IGF-1 and BMP15 genes have been reported as candidate genes because they have a role in growth and skeletal development. It was hypothesized that polymorphism may be present in GH gene, IGF-1, and BMP15 genes which might also be associated with body weight and body measurements in Beetal goat. In the present study, 5 exons of growth hormone (GH) gene, exon 4 and partial region of intron 4 of IGF-1 gene and exon2 of BMP15 gene were amplified from goat genomic DNA.To assess this assumption, a total of 60 adult healthy animals of Beetal goat were selected on the basis of maximum variation in their body weight and body measurement (Body Length, height and heart girth). Animals were divided into two body weight categories, i.e. heavy and low.After blood collection (5ml), genomic DNA was extracted from the whole blood and stored at -20ºC for further use. DNA quantification was performed through agarose gel electrophoresis with the help of standard. PCR conditions were optimized. The genotyping was done through RFLP. General Linear Model (GLM) procedure of the Statistical Analysis System package (SAS Institute Inc, version 9.1) was used to test the effectof genotype on the body weight, body length, body height, and chest girth.Present study resulted in the identification ofsome SNP in GH, IGF-1 candidate genes in Beetalgoats. Genotyping results were monomorphic in GH, and BMP15 genes in animals of studiedbreed, while IGF-1 gene was polymorphic and had significant associationof genotypes with body weight and with body measurements. Genotype AA is significantly associated with low body weight having 38.18 Kg body weight and heart girth 33.65 cm, (P<0.05). AA genotype is also significantly associated with body leangth (P<0.05). The goats having BB genotype is significantly associated with high body weight having body weight 47.13 Kg (P<0.05). Genotype AB is significantly associated with withers height (31.93 cm).The sequencing results of the IGF-1 gene showed conserved and showed no other mutation in studied exonUponsequencing,polymorphism was found in GH gene inhigher and lower body weight animals. One SNP was identified in exon 2. The Non-synonymous substitution mutation (A > G) of CGA to CAA at position 825 caused an amino acid change from threonine into Alanine. While one deletion mutation in intron 4was identifiedat position 1546 in this gene. Up-to our knowledge, this is the first study of growth trait genes in the Beetal goat breed of Pakistan and helpful to understand the genetics of growth genes in Beetal goat breed. It may lead us to the identification of useful molecular markers for future use in the selection of animals with better growth traits.
CONCLUSION
By evaluating the genotyping and sequencingresults of all the target regions of growth hormone GH, IGF-1 and BMP15 genes, it is concluded that GHgene and BMP15 gene in beetal goat are monomorphic. One substitution mutation in exon 2 G>Aat position 825 along with one deletionin intron 4at position 1546 can be used as genetic markers in selection programme of breeding. WhileIGF-1 is of prime importance because IGF-1 gene was polymorphic and had significant association with body weight and measurements.
RECOMMENDATION
Due to limited resources 60 animals were used to determine polymorphism in three growth genesand their association with growth traits. It is recommended for further studies complete gene could be genotyped and sequenced and more number of animals should be used to evaluate these traits in future.
Genetics
2788-T