Mubarka Bano (2015-VA-590)

Effect Of Supplementation Of Probiotic And Phytase Alone And In Combination On The Oxidative Status And Serum Lipid Profile In Japanese Quails - 2017. - 48p.;

Phytase is an enzyme that releases phosphorous from phytate molecule improving the bioavailability of dietary nutrients. Also improves the oxidative status and lower’s serum cholesterol level. Probiotics are live microorganisms given in the diet as a growth promoter. They effect serum lipid profile as well as act as antioxidant. The present study was aimed to evaluate the effect of probiotic and bacterial phytase alone and in combination on serum lipid profile and oxidant and anti-oxidant status of blood, muscle and tissue in Japanese quails.
Two hundred one day old quail chicks were randomly divided into four groups (n=5) with five replicates (n=10). Group-I was fed only basal diet (BD) and named as control group, Group-II was given BD + 0.1% Bacillus cereus. Group-III was given BD+ 0.01% microbial phytase; Group IV was given BD + 0.01% phytase + 0.1% probiotic. On day 28, two birds from each replicate were slaughtered for blood and tissue collection. The parameters to be studied were oxidant and anti-oxidant level in whole blood, muscle and tissue (liver and kidney), Serum lipid profile (TC, TG, HDL and LDL).
Serum total cholesterol concentration was not affected by supplementation. However triglyceride and high density lipoprotein in serum were significantly (P<0.05) affected. Our supplementation causes reduction in triglycerides and increase in high density lipoprotein.
The activity of MDA and catalase in serum and liver tissue was affected significantly (P<0.05), MDA level was reduced and catalase level was increased by supplementation. However our supplementation remained ineffective in muscle MDA and catalase.
Data were presented as mean ± SEM and analyzed by one way-ANOVA technique using SPSS. Group differences were compared using Duncan’s Multiple Range Test at P<0.05.
The present study concluded that probiotic and phytase had shown beneficial effect on lipid profile and oxidative status in Japanese quails. However their synergistic effect was more effective.



Physiology

2842-T


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