Roomana Shams (2014-VA-531)

Effect Of Ethanolic Extract Of Catharanthus Roseus On The Electrophysiology Of Jejunum Infected With Escherichia Coli In Goat - 2017. - 50P.;

Animal products such as meat and milk are the primary source of proteins and amino acid that can be produced throughout the year. Diseases in animals initiate the biophysical and socio economic impact that varies from localized to global. Different pathogens including virus, bacteria and fungi cause different diseases in ruminants. Escherichiacoli are one of the inhabitants of intestinal tract and are responsible for watery diarrhea, bloody diarrhea and hemorrhagic colitis. Diarrheal disease gives rise to global health problem and now a day many plants are used to treat the disease condition. Catharanthus roseusis one of the important medicinal plants used for the treatment of many diseases such as diarrheal, diabetics, wound healing and Alzheimer’s disease. C. roseus maintain the optimum performance of gut barrier by reducing the loss of minerals. The changes in the electrophysiology of jejunum with the addition of C.rouses were measured by Ussing chamber. Objective of this study was to investigate the effects of C.roseus on the barrier function of intestine influenced by E. coli.
Catharanthusroseus may reduce the detrimental effects induced by E.coli and improves the gut barrier function.
Five goats of uniform age and weight were selected and slaughter at local slaughter house. Jejunum was taken from the intestinal part and rinsed with standard buffer. Serosal layer was removed and tissue was mounted in Ussing chamber. After incubation period of 15-20mins different groups were treated with different treatments. Control with no treatment, group B was treated with 10% toxin of E.coli, group C was treated with 1.5% extract of C.rouses, group D was treated with 10% toxin of E.coli and 1.5% extract of C. rouses. After treatment the electrophysiological parameters like Isc and Gt was observed.
C.rouses affected the electrophysiology of isolated jejunum of goats by decreasing the short circuit current which was induced by the E.coli toxin. The decrease of current between the groups is significant (p value < 0.05). ΔIscwas also significant between the groups containing E. coli toxin and C. roseus containing solution. Conductance increased significantly after the addition of E.coli and C. roseus (p value < 0.05).
Data wereanalyzed by using completely randomized ANOVA followed by post-hoc Duncan’s multiple range tests. Level of significance was set at 0.05.
Catharanthus roseus treatment affects the electrophysiological indices of isolated jejunum epithelium of goat presumably by closing the chloride channels and enhances the ions absorption across the jejunum epithelium. This may leads to efficient mineral utilization and better health of animals.



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