Ahmad Usman Zafar
Studies On The Isolation And Serotyuping Of Salmonella From Mesenteric Lymmphnodes Of Sheep And Goats - 1986
Five hundred samples of mesenteric lymph nodes of sheep and goats , 20 from each species , slaughtered at Lahore abattoir, were bacteriologically examined to determine the Salmonella carrier state in healthy sheep and goats. Nine strains of Salmonellae belonging to two serological types i.e. Salmonella typhimurium (l.40%) and Salmonella anatum (0.40%), were isolated. The incidence of Salmonellosis in sheep and goats were recorded as 2.40% and 1.20% respectively.
The media used for primary isolation and purification were Selenite broth, Bismuth sulphite agar, Brillient green agar, MacConkey agar and Nutrient agar. Results of Triple sugar iron agar and Urease test gave a preliminary confirmation of Salmonella organisms. Sugar fermentation reactions, Methyl red and Voges Proskauer tests were also employed for the confirmation of isolates. The Serological identification was carried out with the help of standard antisera manufactured by ' Research Institute of Infectious and Parasitic Diseases Sofia, Bulgaria. Further work on much larger scale is recommended.
Department of Microbiology
0195,T
Studies On The Isolation And Serotyuping Of Salmonella From Mesenteric Lymmphnodes Of Sheep And Goats - 1986
Five hundred samples of mesenteric lymph nodes of sheep and goats , 20 from each species , slaughtered at Lahore abattoir, were bacteriologically examined to determine the Salmonella carrier state in healthy sheep and goats. Nine strains of Salmonellae belonging to two serological types i.e. Salmonella typhimurium (l.40%) and Salmonella anatum (0.40%), were isolated. The incidence of Salmonellosis in sheep and goats were recorded as 2.40% and 1.20% respectively.
The media used for primary isolation and purification were Selenite broth, Bismuth sulphite agar, Brillient green agar, MacConkey agar and Nutrient agar. Results of Triple sugar iron agar and Urease test gave a preliminary confirmation of Salmonella organisms. Sugar fermentation reactions, Methyl red and Voges Proskauer tests were also employed for the confirmation of isolates. The Serological identification was carried out with the help of standard antisera manufactured by ' Research Institute of Infectious and Parasitic Diseases Sofia, Bulgaria. Further work on much larger scale is recommended.
Department of Microbiology
0195,T