Ahsan Rashad, M

Morphological Abnormalities Of Spermatozoa In Pure Bred Friesian, Sahiwal Bulls And Friesian X Sahiwal - 1991

Of the 15 normal semen producing breeding bulls, five bulls from each of pure bred Sahiwal, Friesian and the cross-breds (Friesian X Sahiwal) of the same age group were selected. These bulls were maintained at Semen Production Unit, Qadirabad (Sahiwal) under similar conditions of housing, feeding and management. Two consecutive ejaculates once a week were collected. A total of 395 ejaculates were evaluated for various seminal characteristics, extended, processed and frozen during August, September and October, 1990. During these three months semen parameters like ejaculate volume, mass motility, percent motility and the doses produced per ejaculate declined with increase of ambient temperature and humidity.

The second ejaculate (195) at each collection and for each bull was always taken for studying the sperm morphology. Two slides from each semen sample were prepared in 10% solution of Nigrosin stain. A total of 200 sperm cells were counted in each slide and average values for various morphological abnormalities of spermatozoa in each breed were studied. The sperm cell abnormalities were accounted for head (abnormal, detached), neck (poor attachment, abaxial attachment and fractured neck), thickened mid-piece, protoplasmic droplet (proximal, distal droplet) and tail (detached, coiled- tail-droplet-enclosed, coiled tail).

The mean values of different breeds so obtained for different abnormalities during various months were analysed. The average values for head abnormalities for three breeds were 9.69 ± 1.88, 5.12 ± 060 and 1.56 ± 0.14% in Friesian, cross-bred and Sahiwal, respectively. These abnormalities were highest during August and September in Friesian bulls and lowest in Sahiwal bulls during October.

The neck abnormalities were highest in cross-breds (4.44 ± 0.57%) and lowest in Sahiwal (1.67 ± 0.14%). However, the neck abnormalities were statistically similar during the months of August, September and October. Similar trend of mid-piece abnormality was observed between various breeds and months. Likewise the values of protoplasmic droplets were highest in cross breds (14.12 ± 1.19%) and lowest in Sahiwal (2.36 ± 0.30%). The tail abnormalities were the highest in cross-breds (3.47 ± 0.45%) and lowest in Sahiwal (0.87 ± 0.12%). Among various months the tail abnormalities, were statistically similar (P> 0.05).

The total values of different sperm abnormalities wçre highest in cross-breds during August (30.93 ± 3.11%) and comparatively lower during September and October (28.66 ± 2.28 and 26.11 ± 2.63%), respectively.

Except head abnormalities, all the other abnormalities of spermatozoa were statistically significant among breeds.

Department of Theriogenology


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