Amjad Hussain

A Study To Determine The Time Of Ovulation In Relation To Oestrus In Nili-Ravi Buffalo - 1992

A total of 32 Nili-Ravi cyclic buffaloes were selected for this study. Cyclicity was determined on the basis of presence of corpus luteum and was confirmed by luteal phase levels of progesterone. 10 animals were treated with PGF2-alpha to induce oestrus. The oestrus detection was made by teasing, behavioural symptoins, physical changes and rectal palpation. The receptivity of male was taken as a time for start of oestrus. The ovulation time was determined by repeated palpation and was confirmed by blood progesterone levels. The behavioural signs noted were; acceptance of teaser bull, frequent urination, raised tail, bellowing, restlessness, acceptance of mounting by other females, licking of external genitalia of other animals and loss of appetite. The physical changes recorded were; uterine tone, swollen vulva, mucous discharge on rectal palpation, absence of wrinkles on vulvular lips, hypraemia and wetness in the vagina. There was a little difference in the behavioural symptoms and physical changes among the groups. In the PGF2-alpha treated group the behavioural symptoms and physical changes were slightly more pronounced as compared to natural groups.


The length of oestrus in the natural groups was 23.5 ± 1.5 hours, whereas it was 27.2 ± 2.4 hours in the treatment group. The difference in the length of oestrus among the two groups was statistically significant. The ovulation time in he natural group was 34.6 ± 1.7 hours from the start of oestrus and 11.2 ± 0.5 hours from the end of oestrus. The ovulation time in animals treated with prostaglandin F2-alpha was 39.8 ± 2.9 and 12.6 ± 1 .2 hours in relation to the start and the end of oestrus, respectively. The difference in the ovulation time both in relation to the start and to the end of oestrus between the two groups was significant. The progesterone Values at the time of oestrus were 0. 1 ± 0.02 ng/ml and 0. 1 ± 0. 1 ng/ml in natural and treated groups respectively. And in the luteal phase (13), the progesterone values were 2.1 ± 0.2 and 2.6 ± 1.6 ng/ml in natural and treated groups respectively.



Department of Theriogenology

0240,T


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