Zulfiqar, M
Comparative Studies On The Efficacy Of Indirect Haemagglutination Test (Iha) And Double Immuno Diffusion Test (Did) - 1985
One hundred faecal samples, 100 blood samples and livers including bile ducts were collected from sheep suffering from Fascio1iasis slaughtered at Lahore and Sheikhupura abbatoirs.
Faecal examinations were carried out with fresh smear and centrifugal techniques and only 35 animals were found passing Fasciola ova. Serum was prepared from the blood samples collected from infested sheep for performance of indirect hacinagglutination test and double imniuno-diffusion test. Crude antigen was prepared from the fresh liver flukes specimens collected from infected livers which contained 4.5 mg protein/mi. Hyper immuneerim was prepared in rabbits by inoculating antigen. Double immuno-diffusion test was performed on glass slides coated with 1% agar Gel and. incubated at 37 C for 48 hours. The positive reactions showed diffuse lines. 45 sheep were found positive with Double immuno-diffusion test.. For performing the indirect haemagglutination test fresh red blood cell of the healthy sheep were stabilized with gluteraldehyde and sensitized with Tannic acid. Crude antigen was attached to these cells at pH 6.4 which were used in Indirect haemagglutination test. Serum samples were tested with Fasciola antigen in "V" plates of microtiter sptem. The end points of the plates were read after 16 hours a room temperature. Sora were prediluted 50 times but agglutination was seen even at 1/100 dilution. In positive reactions cells were like mat of cells in the bottom of the wells In negative reactions, cells settle down and looked like button in the center. 59 animal were found positive with indirect haemagglutination test. While comparing the sheep serum samples results with hyper immune serum collected rom rabbits it was found that all the positive sheep serum samples coincide with the results of hyper immune serum.
From the conclusion of the results it was found that indirect haemagglutination test was more sensitive (59%) than double immuno diffusion test(45%)as compared with the coprological examination 35%
Department of Parasitology
0272,T
Comparative Studies On The Efficacy Of Indirect Haemagglutination Test (Iha) And Double Immuno Diffusion Test (Did) - 1985
One hundred faecal samples, 100 blood samples and livers including bile ducts were collected from sheep suffering from Fascio1iasis slaughtered at Lahore and Sheikhupura abbatoirs.
Faecal examinations were carried out with fresh smear and centrifugal techniques and only 35 animals were found passing Fasciola ova. Serum was prepared from the blood samples collected from infested sheep for performance of indirect hacinagglutination test and double imniuno-diffusion test. Crude antigen was prepared from the fresh liver flukes specimens collected from infected livers which contained 4.5 mg protein/mi. Hyper immuneerim was prepared in rabbits by inoculating antigen. Double immuno-diffusion test was performed on glass slides coated with 1% agar Gel and. incubated at 37 C for 48 hours. The positive reactions showed diffuse lines. 45 sheep were found positive with Double immuno-diffusion test.. For performing the indirect haemagglutination test fresh red blood cell of the healthy sheep were stabilized with gluteraldehyde and sensitized with Tannic acid. Crude antigen was attached to these cells at pH 6.4 which were used in Indirect haemagglutination test. Serum samples were tested with Fasciola antigen in "V" plates of microtiter sptem. The end points of the plates were read after 16 hours a room temperature. Sora were prediluted 50 times but agglutination was seen even at 1/100 dilution. In positive reactions cells were like mat of cells in the bottom of the wells In negative reactions, cells settle down and looked like button in the center. 59 animal were found positive with indirect haemagglutination test. While comparing the sheep serum samples results with hyper immune serum collected rom rabbits it was found that all the positive sheep serum samples coincide with the results of hyper immune serum.
From the conclusion of the results it was found that indirect haemagglutination test was more sensitive (59%) than double immuno diffusion test(45%)as compared with the coprological examination 35%
Department of Parasitology
0272,T