Hafeez Khan, A
Induction Of Oestrus In Non Cycling Nili Ravi Buffalo Heifers - 1994
Twenty one non-cycling Nili-Ravi buffalo hcifers with mean (± S.E) age of 31.71 ± 1.32 months and body weight of 417.61 ± 11.26 Kg were randomly divided into three equal groups. Animals in group A were injected 700 IU PMSG intramuscularly. Whereas, animals in group B (n=7) were treated with 1400 IU of PMSG. Animals in group C (n7) were administered 2 CC normal saline, which served as controls. Animals in oestrus were naturally bred with progeny tested bulls. Blood samples were collected before treatment, at oestrus and 10 days after oestrus for plasma progesterone estimation. Rectal palpations were done before treatment, at oestrus, 10 days after oestrus for ovarian structures and on day 60 post breeding for pregnancy diagnosis.
All the buffalo heifers in group A and B exhibited oestrus following treatment with 700 and 1400 IU of PMSG. Three animals from group C (42.85%) showed oestrus. The time from treatment to the onset of oestrus differed non significantly and was 6.14 ± 0.56, 6.00 ± 0.45 and 6.66 ± 0.47 days for groups A, B and C, respectively. No difference was observed towards expression of oestrus symptoms with varying doses of PMSG. The behavioural symptoms and physical changes i.e. frequent urination, acceptance to male, vulvalar sniffing of other females or allowing others to sniff, mucus discharge, vulvalar swelling and uterine tone were found indicative of oestrus in experimental heifers. The duration of oestrus also differed non significantly among the groups. This duration was 22.00 ± 2.01; 27.71 ± 1 .67 and 19.66 ± 0.98 hours for groups A, B and C, respectively.
Three animals from group A (n=7) conceived at induced oestrus. Whereas, no heifer from group B setteled at induced oestrus. Four animals showed next oestrus and two of them conceived. Three animals from group C exhibited oestrus and one of them conceived, rest of the animals remained non cyclic. The overall conception rates were 42.85, 28.57 and 14.28 percent for groups A, B and C, respectively.
Progesterone levels were found representative of ovarian activity in all the groups. Basal levels (> 0.5 ng/ml) of plasma progesterone were observed with smooth ovaries. Higher values for plasma progesterone ranging between 0.95 ± 0.02 to 5.68 ± 0.22 ng/ml were observed in cycling and pregnant animals reprsenting functional corpus lutuem.
Department of Theriogenology
0461,T
Induction Of Oestrus In Non Cycling Nili Ravi Buffalo Heifers - 1994
Twenty one non-cycling Nili-Ravi buffalo hcifers with mean (± S.E) age of 31.71 ± 1.32 months and body weight of 417.61 ± 11.26 Kg were randomly divided into three equal groups. Animals in group A were injected 700 IU PMSG intramuscularly. Whereas, animals in group B (n=7) were treated with 1400 IU of PMSG. Animals in group C (n7) were administered 2 CC normal saline, which served as controls. Animals in oestrus were naturally bred with progeny tested bulls. Blood samples were collected before treatment, at oestrus and 10 days after oestrus for plasma progesterone estimation. Rectal palpations were done before treatment, at oestrus, 10 days after oestrus for ovarian structures and on day 60 post breeding for pregnancy diagnosis.
All the buffalo heifers in group A and B exhibited oestrus following treatment with 700 and 1400 IU of PMSG. Three animals from group C (42.85%) showed oestrus. The time from treatment to the onset of oestrus differed non significantly and was 6.14 ± 0.56, 6.00 ± 0.45 and 6.66 ± 0.47 days for groups A, B and C, respectively. No difference was observed towards expression of oestrus symptoms with varying doses of PMSG. The behavioural symptoms and physical changes i.e. frequent urination, acceptance to male, vulvalar sniffing of other females or allowing others to sniff, mucus discharge, vulvalar swelling and uterine tone were found indicative of oestrus in experimental heifers. The duration of oestrus also differed non significantly among the groups. This duration was 22.00 ± 2.01; 27.71 ± 1 .67 and 19.66 ± 0.98 hours for groups A, B and C, respectively.
Three animals from group A (n=7) conceived at induced oestrus. Whereas, no heifer from group B setteled at induced oestrus. Four animals showed next oestrus and two of them conceived. Three animals from group C exhibited oestrus and one of them conceived, rest of the animals remained non cyclic. The overall conception rates were 42.85, 28.57 and 14.28 percent for groups A, B and C, respectively.
Progesterone levels were found representative of ovarian activity in all the groups. Basal levels (> 0.5 ng/ml) of plasma progesterone were observed with smooth ovaries. Higher values for plasma progesterone ranging between 0.95 ± 0.02 to 5.68 ± 0.22 ng/ml were observed in cycling and pregnant animals reprsenting functional corpus lutuem.
Department of Theriogenology
0461,T