Shafi Ullah Chand
Preparation And Evaluation Of Newcastle Disease Virus (Mesogenic Strain) Oil Based Vaccine - 1998
The present work was proposed to prepare oil based Newcastle disease virus vaccine and to compare its efficacy with imported vaccines. An oil based ND vaccine was prepared using moderately virulent strain of NDV. The virus was cultured in chicken embryos. The allanto-amniotic fluid, chorioallantoic membrane and infected embryo (virus suspension) was subjected to titration. The HA titer of allanto-amniotic fluid (AAF), chorloallantoic membrane (CAM) and embryo was upto 512, 1024 and 2048, respectively. The MD50 was calculated to be 1088/0.1ml.
Effect of temperature on its keeping quality was determined by estimation of its HA potential at various intervals. The AAF was processed for inactivation, sterility and safety tests. Formalin at a rate of 0.12% inactivated the NDV in 48 hours at 37°C. Addition of antibiotic such as gentarnycin and nystatin inhibited common contaminants. An oil based NDV vaccine was prepared by mixing one part of processed AAF in 4 parts of oil base. The oil base contained 4% emulsifier span-80 and 1% tween-80. The vaccine thus prepared from moderately virulent strain was antigenically comparable with the imported ND vaccine. The cost of vaccine production using moderately virulent NDV was Rs.463/bottle (1000 doses) compared to RS.1250/- per bottle of imported vaccine. The price of one ml diagnostic antigen was calculated at Rs.2/ml.
The results of present project encourage to develop an economical and effective oil based ND vaccine and diagnostic HA NDV antigen.
Department of Microbiology
0700,T
Preparation And Evaluation Of Newcastle Disease Virus (Mesogenic Strain) Oil Based Vaccine - 1998
The present work was proposed to prepare oil based Newcastle disease virus vaccine and to compare its efficacy with imported vaccines. An oil based ND vaccine was prepared using moderately virulent strain of NDV. The virus was cultured in chicken embryos. The allanto-amniotic fluid, chorioallantoic membrane and infected embryo (virus suspension) was subjected to titration. The HA titer of allanto-amniotic fluid (AAF), chorloallantoic membrane (CAM) and embryo was upto 512, 1024 and 2048, respectively. The MD50 was calculated to be 1088/0.1ml.
Effect of temperature on its keeping quality was determined by estimation of its HA potential at various intervals. The AAF was processed for inactivation, sterility and safety tests. Formalin at a rate of 0.12% inactivated the NDV in 48 hours at 37°C. Addition of antibiotic such as gentarnycin and nystatin inhibited common contaminants. An oil based NDV vaccine was prepared by mixing one part of processed AAF in 4 parts of oil base. The oil base contained 4% emulsifier span-80 and 1% tween-80. The vaccine thus prepared from moderately virulent strain was antigenically comparable with the imported ND vaccine. The cost of vaccine production using moderately virulent NDV was Rs.463/bottle (1000 doses) compared to RS.1250/- per bottle of imported vaccine. The price of one ml diagnostic antigen was calculated at Rs.2/ml.
The results of present project encourage to develop an economical and effective oil based ND vaccine and diagnostic HA NDV antigen.
Department of Microbiology
0700,T