The Effect Of Experimentally Induced Yolk Sac Infection With Staphylococcus Aureus On Immune Status Of Broiler Chicks
- 2002
The present project was carried out to study the effect of experimentally induced yolk sac infection with Staphylococcus aureus on immune status of broiler chicks. For this purpose one hundred day-old chicks were procured from local market and were randomly divided into two groups, A and B having fifty chicks each. Confirmed pathogenic isolate of Staphylococcus aureus was inoculated into the yolk of each chick of group A while group B acted as control. Ten chicks from each group were slaughtered at interval of 48 hours i.e. on 3rd 5th 7th, and 9th day of the experiment and serum and yolk were collected. Experimental parameters included pathological examination of yolk sac, yolk sac weight/body weight ratio, antibody titres against Newcastle disease virus (NDV) in serum and yolk and analysis of fractional serum proteins.
Results showed that yolk sac weight/body weight ratio was higher in treatment group than in control group. Marked pathological changes (abnormal colour, consistency and odour) were also observed in unabsorbed yolks of the infected group. Geometric mean titres of maternal antibodies against NDV were significantly lower in serum while higher in yolk of the infected group as compared to control one. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used for serum protein analysis and percent area covered by each protein fraction was calculated. The percent area covered by most fractions of serum protein was lower in infected chicks than the control chicks.
It is therefore concluded that experimental yolk sac infection with Saphylococcus aureus leads to decreased maternal antibodies absorption from infected yolks and altered serum proteins indicating decline in the immunity of the chicks.
Department of Pathology
0786,T
The present project was carried out to study the effect of experimentally induced yolk sac infection with Staphylococcus aureus on immune status of broiler chicks. For this purpose one hundred day-old chicks were procured from local market and were randomly divided into two groups, A and B having fifty chicks each. Confirmed pathogenic isolate of Staphylococcus aureus was inoculated into the yolk of each chick of group A while group B acted as control. Ten chicks from each group were slaughtered at interval of 48 hours i.e. on 3rd 5th 7th, and 9th day of the experiment and serum and yolk were collected. Experimental parameters included pathological examination of yolk sac, yolk sac weight/body weight ratio, antibody titres against Newcastle disease virus (NDV) in serum and yolk and analysis of fractional serum proteins.
Results showed that yolk sac weight/body weight ratio was higher in treatment group than in control group. Marked pathological changes (abnormal colour, consistency and odour) were also observed in unabsorbed yolks of the infected group. Geometric mean titres of maternal antibodies against NDV were significantly lower in serum while higher in yolk of the infected group as compared to control one. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used for serum protein analysis and percent area covered by each protein fraction was calculated. The percent area covered by most fractions of serum protein was lower in infected chicks than the control chicks.
It is therefore concluded that experimental yolk sac infection with Saphylococcus aureus leads to decreased maternal antibodies absorption from infected yolks and altered serum proteins indicating decline in the immunity of the chicks.
Department of Pathology
0786,T