Muhammad Jamil

Detection And Chemotherapy Of Sub Clinical Mastitis In Dairy Catlle And Buffaloes - 2008

The aim of this study was to detect subclinical mastitis in bovine and to find out the most appropriate antibiotic for its treatment. For this purpose, Milk samples were taken aseptically from 500 apparently healthy animals (n= 250 cattle and n= 250 buffaloes) for screening tests. Two different tests, Surf Mastitis Test (SMT) and Somatic Cell Count (SCC) were used. Milk samples declared positive by both of the above mentioned tests, were subjected to culture sensitivity test. Six different antibiotics were evaluated i.e. Enrofloxacin, Norfioxacin, Amoxicillin, Oxytetracycline, Gentamicin and penicillin G. On the basis of sensitivity test; two topmost drugs were selected and be given to two equal groups of animals. Each group was comprised of equal number of cattle and buffaloes. Each antibiotic was given for 5 days as intramuscular (IM) injection. A positive and a negative control groups were also kept. To detect the in-vivo efficacy of antibiotic, again the milk samples of all the groups were examined by SMT and SCC on day 10 and day 20 of first injection. The results obtained were the following;
Sub clinical mastitis was found 42.8% in cattle and 37.6% in buffaloes. Out of
the total of 201 mastitis positive milk samples cultured, bacterial growth occurred in
98 (48.75%) of milk samples. The various bacterial species isolated from milk
samples of cattle and buffaloes were E. coli Staphylococcus, Streptococcus, Proteus,
Kiebsiella, Pseudomonas, and Pasteurella with overall percentage of 50 %, 17.34 %,
3.06 %, 12.24%, 10.20%, 2.04% and 5.10 % respectively.

The in vitro efficacy of Enrofloxacin was found to be the best one i.e. 77.55 % followed by Norfioxacin (67.34 %), Gentamicin (53.06 %), Oxytetracyclin (30.61 %), Amoxicillin (22.44 %) and Penicillin G (4.08 %).

After chemotherapy, there was significant difference between treatment groups and control groups (P<0.05). The difference in the efficacy of enrofloxacin and norfioxacin was statistically non significant (P>0.05), however mathematically, the recovery rate by enrofloxacin was greater (93.75%) then norfioxacin (87.50%). Recovery rate was more at day 20 for both antibiotics.

Department of Clinical Medicine & Surgery


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