Waqas Ahmad
Evaluation Of Antiviral And Cytotoxic Activity Of Medicinal Plants Extracts Against Infectious Bursal Disease Virus - 2011
The antiviral activity of plants Glyceriza glabra Linn. (roots), Phyllanthus emblicus Linn. (Fruit), Eugenia jambolana Lam. (Leaves), and Moringa oleifera Lam. (Leaves) were evaluated against Infectious bursal disease virus (IBDV) in this study. Ethanolic extraction of these plants was carried out by using Soxhlet apparatus and extracts was dried by using rotary evaporator. Four dilutions of each extracts viz 100, 50, 25 and 12.5?g/ml were made in distilled water. Vero cells were infected by mild strain of IBDV. Dilutions of these extracts were applied in triplicate manner on Vero cells that are confluent in 96 well cell culture plates. Positive control and negative control for antiviral assay were media plus cells and virus plus media respectively in antiviral assay. A cell culture plate was incubated for four days. After this incubation, viability of cells was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay. The cytotoxic activity of mentioned plant extracts was carried out by treating the cells with mentioned dilutions used in antiviral assay and incubating the 96 well cell culture plate for 4 days. Viability of cells was determined by MTT colorimetric assay. Positive and negative control for cytotoxic evaluation was cells plus media and cells plus media plus DMSO (10 %) respectively. Endpoint of this assay was measured in terms of cell survival percentage. Results were compared for qualitative variables using Chi-square technique and quantitative variables by linear regression analysis. 100 ug/ml and 50 ug/ml concentrations of Moringa oleifera Lam. showed cell survival percentages of 80% and 75% respectively and all four test dilutions of same plant showed no cytotoxicity for Vero cells. Two concentrations of Glycyrrhiza glabra Linn. 25ug/ml and 12.5ug/ml showed prominent cell survival of 75% and 80% respectively and other two concentrations 100ug/ml and 50ug/ml were found cytotoxic. Only 100ug/ml of Phyllanthus emblicus Linn. has shown cytotoxicity and 50ug/ml and 25ug/ml shown prominent antiviral activity. All concentrations of Eugenia jambolana Lam. were found non cytotoxic and 100ug/ml showed some antiviral potential against Infectious Bursal Disease virus.
Department of Pharmaoclogy & Toxicology
1319,T
Evaluation Of Antiviral And Cytotoxic Activity Of Medicinal Plants Extracts Against Infectious Bursal Disease Virus - 2011
The antiviral activity of plants Glyceriza glabra Linn. (roots), Phyllanthus emblicus Linn. (Fruit), Eugenia jambolana Lam. (Leaves), and Moringa oleifera Lam. (Leaves) were evaluated against Infectious bursal disease virus (IBDV) in this study. Ethanolic extraction of these plants was carried out by using Soxhlet apparatus and extracts was dried by using rotary evaporator. Four dilutions of each extracts viz 100, 50, 25 and 12.5?g/ml were made in distilled water. Vero cells were infected by mild strain of IBDV. Dilutions of these extracts were applied in triplicate manner on Vero cells that are confluent in 96 well cell culture plates. Positive control and negative control for antiviral assay were media plus cells and virus plus media respectively in antiviral assay. A cell culture plate was incubated for four days. After this incubation, viability of cells was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay. The cytotoxic activity of mentioned plant extracts was carried out by treating the cells with mentioned dilutions used in antiviral assay and incubating the 96 well cell culture plate for 4 days. Viability of cells was determined by MTT colorimetric assay. Positive and negative control for cytotoxic evaluation was cells plus media and cells plus media plus DMSO (10 %) respectively. Endpoint of this assay was measured in terms of cell survival percentage. Results were compared for qualitative variables using Chi-square technique and quantitative variables by linear regression analysis. 100 ug/ml and 50 ug/ml concentrations of Moringa oleifera Lam. showed cell survival percentages of 80% and 75% respectively and all four test dilutions of same plant showed no cytotoxicity for Vero cells. Two concentrations of Glycyrrhiza glabra Linn. 25ug/ml and 12.5ug/ml showed prominent cell survival of 75% and 80% respectively and other two concentrations 100ug/ml and 50ug/ml were found cytotoxic. Only 100ug/ml of Phyllanthus emblicus Linn. has shown cytotoxicity and 50ug/ml and 25ug/ml shown prominent antiviral activity. All concentrations of Eugenia jambolana Lam. were found non cytotoxic and 100ug/ml showed some antiviral potential against Infectious Bursal Disease virus.
Department of Pharmaoclogy & Toxicology
1319,T