Asif Nadeem
Genome-Wide Association Mapping To Approach The Candidate Gene Having Potential Role In Dairy Bull Fertility - 2011
Reproductive efficiency is a most important determinant of dairy profitability. Fertility in
the herd is absolutely critical for both male and female animals. Fertility studies in dairy
cattle were directed toward the female side and very little importance has been placed on
the influence of the service bull. In this study association mapping was carried out Cor
fertility trait in Holstein dairy cattle bulls using high-throughput and a high-density SNi>
genotyping array. Single nucleotide polymorphisms (SNPs) were associated with dair,
cattle bull fertility. Associated SNPs were queried in the bovine genome. Seven SNPs
were found within the genes and fourteen were within 10 kh o! a gene. Seven gl'nes.
namely LEPRELl, MOBKL3, CD247, LRRC8J\, LRFN5, IT] I [J and [·.NTP[) J were
seleeted as candidate genes. Resequencing and fine mapping of selected candidate genes
were performed and identified SNPs were associated with dairy cattle hull fertility. This
is the first GWA study for dairy bull fertility using the Illumina Bovine S~ P50 Bcadchip
containing 54001 S Ps powered by I1lumina lnfinium-Il assay.
Institute of Biochemistry & Biotechnology
Phd. thesis
1354,T
Genome-Wide Association Mapping To Approach The Candidate Gene Having Potential Role In Dairy Bull Fertility - 2011
Reproductive efficiency is a most important determinant of dairy profitability. Fertility in
the herd is absolutely critical for both male and female animals. Fertility studies in dairy
cattle were directed toward the female side and very little importance has been placed on
the influence of the service bull. In this study association mapping was carried out Cor
fertility trait in Holstein dairy cattle bulls using high-throughput and a high-density SNi>
genotyping array. Single nucleotide polymorphisms (SNPs) were associated with dair,
cattle bull fertility. Associated SNPs were queried in the bovine genome. Seven SNPs
were found within the genes and fourteen were within 10 kh o! a gene. Seven gl'nes.
namely LEPRELl, MOBKL3, CD247, LRRC8J\, LRFN5, IT] I [J and [·.NTP[) J were
seleeted as candidate genes. Resequencing and fine mapping of selected candidate genes
were performed and identified SNPs were associated with dairy cattle hull fertility. This
is the first GWA study for dairy bull fertility using the Illumina Bovine S~ P50 Bcadchip
containing 54001 S Ps powered by I1lumina lnfinium-Il assay.
Institute of Biochemistry & Biotechnology
Phd. thesis
1354,T