Faiza Maqbool

Association Of Myogenic Factor 5 (Myf5) Gene Polymophism With Meat Quantity And Quality Traits In Sahiwal - 2012

Pakistan has a large population of farm animals, which are consuming for many purposes. Livestock is the major source of money for any country. Livestock is the major machines and factories which convert roughage (grasses and shrubs etc.) into quality food i.e. Meat and milk.
The Myf5 gene is a member of basic helix-loop-helix family of transcription factors which is involved in the regulation of myogenesis. Its main role in muscle growth, development, proliferation, muscle fibers formation and muscle functioning makes it a candidate gene for molecular marker of meat production in livestock.
Myf5 gene in cattle has been mapped to chromosome 5 and has a length of 3236 bp (Gen Bank accession no. NC-007303). It consists of three exons and two introns. Exons have the lengths of 659, 76 and 1245bp. Role of Myf5 gene in muscle development and growth makes it a candidate gene for meat production in farm animals. In this study association of myogenic factor 5 (Myf5) gene polymorphism with meat quality and quantity traits in Sahiwal cattle was checked out.
In this study blood samples were collected from Sahiwal cattle breeds and DNA was extracted from leukocytes. DNA amplification was done by PCR. Then sequencing of amplified gene was done by Genetic Sequencer.
Allele frequencies and genotype frequencies were statistically analyzed by using SNPator software. The relationship between SNP marker genotypes of myogenic factor 5 (Myf5) gene with meat quality and quantity traits was evaluated by using SNPator software. This study will be a helpful tool for marker assisted selection of beef cattle.

Department of Master of Philosophy


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