Sadaf Anwar

Comparative Efficacy Of Different Diagnostic Techniques For Ovine Haemonchosis Through Faecal - 2013

Livestock plays pivotal role in the economy of country and small ruminants are the major source of food products for human. Haemonchus contortus is the most significant parasite of small ruminants and cause heavy production losses by causing reduction in meat and wool production. The parasite directly affects the health of an animal and causes anemia, hemorrhages, anorexia, weight loss and death of affected animal.
This study was designed to diagnose the Haemonchosis in sheep in and around Lahore. The accurate diagnoses of the parasite are important for its control and treatment. Direct smear, floatation technique and Polymerase Chain Reaction (PCR) were applied to check the sensitivity and specificity in diagnosing Haemonchosis in sheep. For this purpose 100 faecal samples were collected randomly from different areas of Lahore. Each faecal sample was examined by direct smear method and floatation technique. Out of 100 faecal samples 44 were microscopically positive. 30 by direct smear method and 44 by floatation technique.
Specific primers were designed to diagnose Haemonchosis in sheep by using ITS-2 Region. 44 microscopically positive samples were confirmed by PCR. 29 (66%) samples were found to be PCR-positive and 15 (34%) were found to be PCR-negative.
Several other species of parasites were also found during microscopic examination of faecal samples. Two samples wetre found positive for coccidial oocyst and seven samples were also positive for other nematodes along with Haemonchus spp.
Flotation technique was found to be superior in diagnosis of Haemonchosis as compared to direct smear.
From current study it is concluded that Haemonchosis is widely prevalent in ovines. Its accurate diagnosis is essential for the treatment of infectious diseases and control of this parasite. Molecular technique has the advantage over conventional diagnostic techniques because PCR is more specific than conventional methods of diagnosis. According to the present study by conventional method there is 34% error chances to diagnose other Trichstrongylid eggs as Haemochus spp. The main advantage of using PCR as diagnostic test, are an increased speed of diagnosting the disease and its capability to notice low worm burden in small volume of faeces from individual animals.

Department of Parasitology


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