Muhammad Javed Iqbal
Effect Of Eugenia Jambolana (Jamun) Seeds And Leaves Etract On Angiogenesis - 2013
The present study was designed to explore the effects of aqueous and ethanolic extract of leaves and seeds of Eugenia jambolana on angiogenesis by means of the chicken chorioallentoic membrane assay. The Eugenia jambolana was collected from the locality of Lahore. Sequential dilutions were made with distilled water. Eggs were taken from local hatchery and divided into 4 groups. Each group was comprised of five eggs. The eggs were incubated at 37 C0. At day 4, 3-4 ml albumin was removed with the help of syringe. At day 5, sample solutions of various concentrations were applied to chorioallentoic membrane (CAM). After 24 hours, the change in CAM was observed by taking the images. SPIP was utilized for computerized measurements of the diameter of blood vessels and CAM. Overall area of the CAM was also drastically minimized among all groups treated with sample solutions.
The data was analyzed for statistical analysis by using SPSS software (version 13 for windows). ANOVA was performed to determine significance among different groups. The value of P<0.05 was considered as significant.
Different extracts of Eugenia Jambolana exhibited anti-angiogenic potential in the following order. WS > WL = ES > EL. In case of WS 4% solution area of CAM is 285.66 mm2, WL 4% 586.77 mm2, ES 4% 567.47 mm2 and EL 4% 634.23 mm2 while in case of negative control area of CAM 2473 mm2 . The extracts exhibited anti-angiogenic potential in variable magnitude. Dilutions of water extract of seeds exhibited most anti-angiogenic potential (p<0.01) in comparison to other treated groups. Collectively, these observations show that extract of Eugenia jambolana seeds and leaves contains polar compounds that ceased growth, primary vessel formation and cell propagation in CAMs.
Department of Pharmaoclogy & Toxicology
1830,T
Effect Of Eugenia Jambolana (Jamun) Seeds And Leaves Etract On Angiogenesis - 2013
The present study was designed to explore the effects of aqueous and ethanolic extract of leaves and seeds of Eugenia jambolana on angiogenesis by means of the chicken chorioallentoic membrane assay. The Eugenia jambolana was collected from the locality of Lahore. Sequential dilutions were made with distilled water. Eggs were taken from local hatchery and divided into 4 groups. Each group was comprised of five eggs. The eggs were incubated at 37 C0. At day 4, 3-4 ml albumin was removed with the help of syringe. At day 5, sample solutions of various concentrations were applied to chorioallentoic membrane (CAM). After 24 hours, the change in CAM was observed by taking the images. SPIP was utilized for computerized measurements of the diameter of blood vessels and CAM. Overall area of the CAM was also drastically minimized among all groups treated with sample solutions.
The data was analyzed for statistical analysis by using SPSS software (version 13 for windows). ANOVA was performed to determine significance among different groups. The value of P<0.05 was considered as significant.
Different extracts of Eugenia Jambolana exhibited anti-angiogenic potential in the following order. WS > WL = ES > EL. In case of WS 4% solution area of CAM is 285.66 mm2, WL 4% 586.77 mm2, ES 4% 567.47 mm2 and EL 4% 634.23 mm2 while in case of negative control area of CAM 2473 mm2 . The extracts exhibited anti-angiogenic potential in variable magnitude. Dilutions of water extract of seeds exhibited most anti-angiogenic potential (p<0.01) in comparison to other treated groups. Collectively, these observations show that extract of Eugenia jambolana seeds and leaves contains polar compounds that ceased growth, primary vessel formation and cell propagation in CAMs.
Department of Pharmaoclogy & Toxicology
1830,T