Muhammad Awais (2009-VA-539)
Development Of Dna Based Diagnosis Of Babesia Canis In Dogs And Its Specificity With Peripheral Blood Smear Microscopy - 2015. - 75p.;
Babesia canis (B. canis) is an intra-erythrocytic parasite and it is responsible for canine babesiosis. Mainly ticks are responsible for its transmission to vertebrate host. Currently, there are three sub-species of B. canis has been identified i.e. B. caniscanis(B. c. canis), B. canis vogeli (B. c. vogeli)and B. canis rossi (B. c. rossi).Clinical presentation and severity of infection are markedly different for each sub-specie. Routinely used methods for the diagnosis of canine babesiosis include clinical presentation, host/vector specificity, blood smear microscopy and Immunological testing (IFAT and ELISA).
In this study, blood smear microscopy and molecular based technique, PCR, were evaluated. This study has comparative as well as developmental nature.Peripheral blood smear microscopy is cost effective and quick method of diagnosis. But the limitations associated with microscopy include low parasitaemia in chronic and asymptomatic infection, morphological similarity of B. canis with other species of Plasmodium and Theileria. Furthermore, personal expertise in morphology based identification and slide preparation may also hamper the precise identification. These limitations may lead to misdiagnose the infection.
PCR based method, developed in this study, found to be more specific and sensitive than conventional microscopy. Fifty blood samples were collected from September, 2014 to November, 2014. These samples were screened microscopically as well as with PCR. B. canis-like bodies were identified in forty-two (42) samples. But all (50) samples were found positive for B. canis¬via PCR. Sequencing results following PCR have shown that identified sub-specie is similar withB. c. vogeli(99%).
This study clearly depict the likelihood of misdiagnosis with microscopy due to morphological similarities with other piroplasms. Therefore, PCR based methodology was found highly specific for B. canis and produced high throughput. Furthermore, sub-species differentiation is only possible with molecular based approach. As the described approach is found highly specific and sensitive than other conventional methods, therefore, it may aid in the development of clear canine babesiosis geographical distributional picture. Which ultimately help in the development of control and treatment strategies which may eradicate babesiosis.
Institute of Biochemistry and Biotechnology
Molecular Biology and Biotechnology
2287-T
Development Of Dna Based Diagnosis Of Babesia Canis In Dogs And Its Specificity With Peripheral Blood Smear Microscopy - 2015. - 75p.;
Babesia canis (B. canis) is an intra-erythrocytic parasite and it is responsible for canine babesiosis. Mainly ticks are responsible for its transmission to vertebrate host. Currently, there are three sub-species of B. canis has been identified i.e. B. caniscanis(B. c. canis), B. canis vogeli (B. c. vogeli)and B. canis rossi (B. c. rossi).Clinical presentation and severity of infection are markedly different for each sub-specie. Routinely used methods for the diagnosis of canine babesiosis include clinical presentation, host/vector specificity, blood smear microscopy and Immunological testing (IFAT and ELISA).
In this study, blood smear microscopy and molecular based technique, PCR, were evaluated. This study has comparative as well as developmental nature.Peripheral blood smear microscopy is cost effective and quick method of diagnosis. But the limitations associated with microscopy include low parasitaemia in chronic and asymptomatic infection, morphological similarity of B. canis with other species of Plasmodium and Theileria. Furthermore, personal expertise in morphology based identification and slide preparation may also hamper the precise identification. These limitations may lead to misdiagnose the infection.
PCR based method, developed in this study, found to be more specific and sensitive than conventional microscopy. Fifty blood samples were collected from September, 2014 to November, 2014. These samples were screened microscopically as well as with PCR. B. canis-like bodies were identified in forty-two (42) samples. But all (50) samples were found positive for B. canis¬via PCR. Sequencing results following PCR have shown that identified sub-specie is similar withB. c. vogeli(99%).
This study clearly depict the likelihood of misdiagnosis with microscopy due to morphological similarities with other piroplasms. Therefore, PCR based methodology was found highly specific for B. canis and produced high throughput. Furthermore, sub-species differentiation is only possible with molecular based approach. As the described approach is found highly specific and sensitive than other conventional methods, therefore, it may aid in the development of clear canine babesiosis geographical distributional picture. Which ultimately help in the development of control and treatment strategies which may eradicate babesiosis.
Institute of Biochemistry and Biotechnology
Molecular Biology and Biotechnology
2287-T