Faisal Sher (2007-VA-11)
Cross Sectional Study Of Newcastle Disease Virus In Wild Captive Peacocks (Pavo Cristatus) In Zoological Gardens Of District Lahore - 2015. - 51p.;
Newcastle disease virus (NDV) is RNA virus.It is the member of avian paramyxovirus type 1 (APMV-1).Its genus is Avulavirus genus. The incubation period ranges from 2-15 days. NDV strains are classified into velogenic, mesogenic and lentogenic categories. Newcastle disease is a virus disease of birds characterized by variable combinations of gastroenteritis respiratory distress and nervous signs.
A cross sectional study was conducted for the duration of 3 months in order to identify the prevalence of Newcastle disease in five zoological gardens of Lahore district. Peacocks were restrained by the trained persons and oropharyngealsamples were collected from apparently healthy Peacocks present in the zoological gardens in Lahore. Sampling of 200Peacocks was done by convenience sampling and stored in freezer at -80°C for further analysis.Virus isolation by egg inoculation was performed to isolate virus and confirmationof (NDV) Newcastle disease virus was done by conducting HI test with specific antisera.Samples were inoculated in 10 days embryonated hen’s eggsand allantoic fluid was collected and tested for haemagglutination (HA) activity. Positive samples were confirmed by haemagglutination inhibition (HI) test.
Frequency distribution through age, sex, breed, origin, date and site of collection were examined by standard statistical methods to determine the prevalence by virus isolation method in that specified population. The data was analyzed by using two way contingency tables with Chi-square test for association. Test was performed to check the association of NDV with age, sex, breeds, origin, and specimen type. The significance level was kept at p<0.05. 2 samples were found positive. Overall prevalence in wild captive peacocks was 1% (95 CI=.1-3.6). 200 samples were screened by spot HA test and 198 samples were found negative in four zoological gardens while 2 samples that were positive found in private zoo samples. Significant association was found between positive samples for NDV and zoological gardens.
Prevalence estimates of (NDV) Newcastle disease virus was generated for Peacock population. Associated factors were identified through this study. Results were shared with international community working for the control and eradication of Newcastle disease.
Department of Epidemiology and Public Health
2411-T
Cross Sectional Study Of Newcastle Disease Virus In Wild Captive Peacocks (Pavo Cristatus) In Zoological Gardens Of District Lahore - 2015. - 51p.;
Newcastle disease virus (NDV) is RNA virus.It is the member of avian paramyxovirus type 1 (APMV-1).Its genus is Avulavirus genus. The incubation period ranges from 2-15 days. NDV strains are classified into velogenic, mesogenic and lentogenic categories. Newcastle disease is a virus disease of birds characterized by variable combinations of gastroenteritis respiratory distress and nervous signs.
A cross sectional study was conducted for the duration of 3 months in order to identify the prevalence of Newcastle disease in five zoological gardens of Lahore district. Peacocks were restrained by the trained persons and oropharyngealsamples were collected from apparently healthy Peacocks present in the zoological gardens in Lahore. Sampling of 200Peacocks was done by convenience sampling and stored in freezer at -80°C for further analysis.Virus isolation by egg inoculation was performed to isolate virus and confirmationof (NDV) Newcastle disease virus was done by conducting HI test with specific antisera.Samples were inoculated in 10 days embryonated hen’s eggsand allantoic fluid was collected and tested for haemagglutination (HA) activity. Positive samples were confirmed by haemagglutination inhibition (HI) test.
Frequency distribution through age, sex, breed, origin, date and site of collection were examined by standard statistical methods to determine the prevalence by virus isolation method in that specified population. The data was analyzed by using two way contingency tables with Chi-square test for association. Test was performed to check the association of NDV with age, sex, breeds, origin, and specimen type. The significance level was kept at p<0.05. 2 samples were found positive. Overall prevalence in wild captive peacocks was 1% (95 CI=.1-3.6). 200 samples were screened by spot HA test and 198 samples were found negative in four zoological gardens while 2 samples that were positive found in private zoo samples. Significant association was found between positive samples for NDV and zoological gardens.
Prevalence estimates of (NDV) Newcastle disease virus was generated for Peacock population. Associated factors were identified through this study. Results were shared with international community working for the control and eradication of Newcastle disease.
Department of Epidemiology and Public Health
2411-T