Uzma Sarwar (2014-VA-777)

Development Of Dna Based Diagnosis Of Theileriosis In Cattle And Its Specificity With Blood Smear Microscopy - 2016. - 75p.;

Theileria annulata and Theileria parva are intra-erythrocytic parasites which are responsible for
causing tropical theileriosis and East Coast fever in cattle respectively. This parasite is
transmitted by ticks to vertebrate host i.e. cattle. Currently used diagnostic methods for diagnosis
of bovine theileriosis are clinical symptoms, peripheral blood smear microscopy and serological
tests (IFAT and ELISA).
Current study was conducted to compare the specificity and sensitivity of blood smear
microscopy and PCR techniques to diagnose bovine theileriosis. This study is comparative as
well as developmental in nature. Although peripheral blood smears microscopy is cost effective
and quick method of diagnosis in case of high or moderate parasitaemia in blood. But the
limitations associated with microscopy include false negative diagnosis in case of low
parasitaemia in chronic and asymptomatic infection, morphological similarity of Theileria with
other species of Plasmodium and Babesia. These limitations may lead to misdiagnose the
infection due to which disease may remain unnoticed. PCR based method, developed in this
study, and is found to be more specific and sensitive than conventional microscopy. Fifty blood
samples were collected from September, 2015 to November, 2015. These samples were screened
microscopically as well as with PCR for presence of Theileria. Nine samples were found to be
positive microscopically but 18 samples were found positive by PCR. The results obtained from
the study clearly show that PCR is more reliable, precise and sensitive assay for diagnosis of
bovine theileriosis then microscopy. It is hoped that proposed method to diagnose Theileria will
help to nullify the problems associated with microscopy. This will ultimately facilitate in the
formulation of effective treatment control and vaccine development strategies.

Molecular Biology and Biotechnology


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