Ghulam Abbas (2011-VA-748)
Molecular Phylogeny And Diversity Analysis Of Bovidae (Boselaphus Tragocamelus, Antilope Cervicapra) And Cervidae (Axis Axis, Axis Porcinus) In Pakistan - 2016. - 134p.;
Many species of mammals have declined within the past two centuries due to human
caused disturbances and the unsustainable use of natural resources. Molecular methods have an
important role in phylogeny and diversity analysis. The present study was designed for diversity
analysis of Boselaphus tragocamelus & Antelope cervicapra (Bovidae) and Axis axis & Axis
porcinus (Cervidae) family in Pakistan. A total of 25 samples from each of the four species were
collected from different parks, zoos and natural habitats. DNA was extracted, PCR primers were
designed and cytochrome-b, cytochrome-c gene and d-loop regions were amplified by PCR.
PCR products were sequenced bi-directionally by Big DyeTM Terminator. Bioinformatics tools,
Blast 2 sequences, Clustal-W, MEGA-6, Bioconductor in “R” were applied for analysis. The
clustering of the samples indicates that each species contains less within-population genetic
variability. Same pattern was observed when sequence of three genes was combined and MDS
plot was constructed. Phylogenetic analysis of the gene sequences revealed that each species
comprised a clade that is clearly distinct from the clade comprised of other species of deer
selected for this study. Finding of this study indicated that these species of deer have significant
genetic variations among-species that differentiate them from each other. This is the first report
from our region. The information of selected species of deer is prerequisite for designing
effective strategy in future conservation practices. However further genomic investigations
should be carried out at larger scale.
Molecular Biology and Biotechnology
IBBT
Phd thesis
2560-T
Molecular Phylogeny And Diversity Analysis Of Bovidae (Boselaphus Tragocamelus, Antilope Cervicapra) And Cervidae (Axis Axis, Axis Porcinus) In Pakistan - 2016. - 134p.;
Many species of mammals have declined within the past two centuries due to human
caused disturbances and the unsustainable use of natural resources. Molecular methods have an
important role in phylogeny and diversity analysis. The present study was designed for diversity
analysis of Boselaphus tragocamelus & Antelope cervicapra (Bovidae) and Axis axis & Axis
porcinus (Cervidae) family in Pakistan. A total of 25 samples from each of the four species were
collected from different parks, zoos and natural habitats. DNA was extracted, PCR primers were
designed and cytochrome-b, cytochrome-c gene and d-loop regions were amplified by PCR.
PCR products were sequenced bi-directionally by Big DyeTM Terminator. Bioinformatics tools,
Blast 2 sequences, Clustal-W, MEGA-6, Bioconductor in “R” were applied for analysis. The
clustering of the samples indicates that each species contains less within-population genetic
variability. Same pattern was observed when sequence of three genes was combined and MDS
plot was constructed. Phylogenetic analysis of the gene sequences revealed that each species
comprised a clade that is clearly distinct from the clade comprised of other species of deer
selected for this study. Finding of this study indicated that these species of deer have significant
genetic variations among-species that differentiate them from each other. This is the first report
from our region. The information of selected species of deer is prerequisite for designing
effective strategy in future conservation practices. However further genomic investigations
should be carried out at larger scale.
Molecular Biology and Biotechnology
IBBT
Phd thesis
2560-T