Waqar Azeem (2014-VA-06)
Detoxification Of Aflatoxins By Leaves Extract Of Indigenous Plants And Their Biological Evaluation - 2016. - 47p.;
Aflatoxins contamination in poultry feed is a major problem in Pakistan. It is responsible for economic loss to poultry industry and a threat to human health trough the consumption of infected meat. There are some conventional methods in use to detoxify aflatoxins from feed but they do not fully obey drug and food regulations. Plant extracts have a potential to become a cost effective and non-toxic method in this regard. Leaves extracts of various indigenous plants have the potential to detoxify aflatoxin contaminated feed and making it safe for animal consumption. Airdried leaves of neem(Azadiricha indica), kikar(Acacia nilotica) and jaman (Eugenia jambolana) were extracted in methanol and filtered to remove undissolved particles. Filtrate was evaporated to remove excess solvent and it was mixed with the already grown aflatoxin on rice from spore suspension of Aspergillusflavus. Aflatoxin levels were measured using HPLC method and percentage inhibition was calculated. Broiler chicks were divided into groups and given leaves extract treated and untreated feed accordingly. The difference between their mortality, growth rate and feed conversion ratio (FCR) was monitored before and after the trials. The data collected from these observations was analyzed by applying one-way analysis of variance. Use of leaves extracts for aflatoxin detoxification is a simple and cost effective detoxification method which seems to offer good prospective by detoxifying the animal feed, reducing economic losses and health related serious risks.
Biochemistry
2621-T
Detoxification Of Aflatoxins By Leaves Extract Of Indigenous Plants And Their Biological Evaluation - 2016. - 47p.;
Aflatoxins contamination in poultry feed is a major problem in Pakistan. It is responsible for economic loss to poultry industry and a threat to human health trough the consumption of infected meat. There are some conventional methods in use to detoxify aflatoxins from feed but they do not fully obey drug and food regulations. Plant extracts have a potential to become a cost effective and non-toxic method in this regard. Leaves extracts of various indigenous plants have the potential to detoxify aflatoxin contaminated feed and making it safe for animal consumption. Airdried leaves of neem(Azadiricha indica), kikar(Acacia nilotica) and jaman (Eugenia jambolana) were extracted in methanol and filtered to remove undissolved particles. Filtrate was evaporated to remove excess solvent and it was mixed with the already grown aflatoxin on rice from spore suspension of Aspergillusflavus. Aflatoxin levels were measured using HPLC method and percentage inhibition was calculated. Broiler chicks were divided into groups and given leaves extract treated and untreated feed accordingly. The difference between their mortality, growth rate and feed conversion ratio (FCR) was monitored before and after the trials. The data collected from these observations was analyzed by applying one-way analysis of variance. Use of leaves extracts for aflatoxin detoxification is a simple and cost effective detoxification method which seems to offer good prospective by detoxifying the animal feed, reducing economic losses and health related serious risks.
Biochemistry
2621-T