Muhammad Balal Munawar (2014-VA-516)
Effect Of Post Pelleting Liquid Phytase Supplementation On Growth Performance And Phosphorous Utilization In Broilers - 2016. - 49p.;
Poultry sector is one of the most developed and vibrant segment of agriculture sector in
Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200
billion rupees investment and offering employment directly or indirectly to 1.5 million people. In
vegetable sources used in poultry rations, P is mostly present in phytate form. Phytate P is not
digested by poultry due to lack of endogenous phytase enzyme. So exogenous phytase are now
added in feed to release P from phytate. Phytase is mainly derived from fungi (Aspergilus niger)
and bacteria (Escherichi coli). Phytase starts work either at 3rd C (3-Phytase) or at 6thC (6-Phytase)
of phytate Phytase mostly used in pre pelleting powder form but it has some activity issues during
high pelleting temperature. Steam application is the most important factor that reduces enzyme
activity due high steam temperature and addition of moisture ultimately lead to enzyme inactivity.
Post pelleting liquid enzyme can be one solution to combat enzyme activity issue.
In total, 504 1-d-old chicks were procured from a commercial hatchery and randomly
assigned to six dietary treatments with Hubbard straight run n=84 chicks per treatment. Each
treatment was further divided into 3 replicates with 28 birds/replicate. One treatment was served
as positive control, while second was served as negative control with (0.30 and 0.26 %) P in starter
and grower respectively. Other four diets had two levels (500 and 750 FTU/kg) of each powder
and liquid enzyme. Liquid enzyme supplementation was done post pelleting. Pelleting temperature
was kept at 85o C.
Feed intake, body weight gain and FCR was recorded at the end of each week. At 35th day of
experiment, 3 birds per replicate were selected randomly, weighed and slaughtered. At the time of
slaughtering, blood samples were collected in heparinized tubes for the determination of plasma
SUMMARY
40
P.Ileal digesta from the slaughtered birds was collected for the determination of P digestibility.
The visceral organs and left tibia from the slaughtered birds were collected, organ weight was
recorded and tibias were ashed for determination of Calcium and P.
Data collected was analyzed using analysis of variance (ANOVA) technique under
Completely Randomized Design (CRD) and means were compared through DMR test by
using SAS 9.1 version (SAS 2001).
The conducted study showed 49.45%, 64.64%, and 8.64 % higher results in feed
intake, body weight gain and FCR when best group H compared with group A (NC). Liver
and heart weight increase in group A, however gizzard weight showed non significant
(p>0.05) results among all groups. Tibia ash, Phosphorous, calcium and plasma P showed
significant results (p<0.05) when all phytase supplemented diets compared with NC.
Animal Nutrition
2631-T
Effect Of Post Pelleting Liquid Phytase Supplementation On Growth Performance And Phosphorous Utilization In Broilers - 2016. - 49p.;
Poultry sector is one of the most developed and vibrant segment of agriculture sector in
Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200
billion rupees investment and offering employment directly or indirectly to 1.5 million people. In
vegetable sources used in poultry rations, P is mostly present in phytate form. Phytate P is not
digested by poultry due to lack of endogenous phytase enzyme. So exogenous phytase are now
added in feed to release P from phytate. Phytase is mainly derived from fungi (Aspergilus niger)
and bacteria (Escherichi coli). Phytase starts work either at 3rd C (3-Phytase) or at 6thC (6-Phytase)
of phytate Phytase mostly used in pre pelleting powder form but it has some activity issues during
high pelleting temperature. Steam application is the most important factor that reduces enzyme
activity due high steam temperature and addition of moisture ultimately lead to enzyme inactivity.
Post pelleting liquid enzyme can be one solution to combat enzyme activity issue.
In total, 504 1-d-old chicks were procured from a commercial hatchery and randomly
assigned to six dietary treatments with Hubbard straight run n=84 chicks per treatment. Each
treatment was further divided into 3 replicates with 28 birds/replicate. One treatment was served
as positive control, while second was served as negative control with (0.30 and 0.26 %) P in starter
and grower respectively. Other four diets had two levels (500 and 750 FTU/kg) of each powder
and liquid enzyme. Liquid enzyme supplementation was done post pelleting. Pelleting temperature
was kept at 85o C.
Feed intake, body weight gain and FCR was recorded at the end of each week. At 35th day of
experiment, 3 birds per replicate were selected randomly, weighed and slaughtered. At the time of
slaughtering, blood samples were collected in heparinized tubes for the determination of plasma
SUMMARY
40
P.Ileal digesta from the slaughtered birds was collected for the determination of P digestibility.
The visceral organs and left tibia from the slaughtered birds were collected, organ weight was
recorded and tibias were ashed for determination of Calcium and P.
Data collected was analyzed using analysis of variance (ANOVA) technique under
Completely Randomized Design (CRD) and means were compared through DMR test by
using SAS 9.1 version (SAS 2001).
The conducted study showed 49.45%, 64.64%, and 8.64 % higher results in feed
intake, body weight gain and FCR when best group H compared with group A (NC). Liver
and heart weight increase in group A, however gizzard weight showed non significant
(p>0.05) results among all groups. Tibia ash, Phosphorous, calcium and plasma P showed
significant results (p<0.05) when all phytase supplemented diets compared with NC.
Animal Nutrition
2631-T