Molecular Characterization Of Canine Babesiosis In Ticks And Dogs (Record no. 10369)

000 -LEADER
fixed length control field 02499nam a22002057a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20170126121856.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 170126b2016 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2642-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Tahira Sarwar (2014-VA-523)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Wasim Shehzad
245 ## - TITLE STATEMENT
Title Molecular Characterization Of Canine Babesiosis In Ticks And Dogs
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2016.
300 ## - PHYSICAL DESCRIPTION
Number of Pages 61p.;
502 ## - DISSERTATION NOTE
Dissertation note Babesia canis is an intra-erythrocytic parasite which cause canine babesiosis in both animals and humans. Currently, there are three sub-species of Babesia canis has been identified i.e Babesia canis canis , Babesia canis vogeli and Babesia canis rossi. Currently used diagnostic methods are clinical symptoms, peripheral blood smear microscopy and serological tests (IFAT and ELISA).Current study was conducted to compare the specificity and sensitivity of blood smear microscopy and PCR techniques to diagnose canine babesiosis. This study is comparative as well as developmental in nature. Although peripheral blood smear microscopy is cost effective and quick method of diagnosis in case of high or moderate parasitaemia in blood. But the limitations associated with microscopy include false negative diagnosis in case of low parasitaemia in chronic and asymptomatic infection,morphological similarity of Babesia with other species of Plasmodium and Theileria these limitations may lead to misdiagnose the infection due to which disease may remain unnoticed.Total 50 samples comprising of 25 blood samples and 25 ticks were collected randomly from infected dogs from June, 2015 to November, 2015. These samples were screened microscopically as well as with PCR. Out of 50 samples of dogs and ticks, 18 samples found to be positive for the Babesia canis. 11 samples are Babesia canis vogeli and 07 samples are Babesia canis canis were to be identified in positive samples of dogs and ticks.The results obtained from the study clearly show that PCR is more reliable, precise and sensitive assay for diagnosis of canine babesiosis then microscopy. It is hoped that proposed method to diagnose babesiosis will help to nullify the problems associated with microscopy. This will ultimately facilitate in the formulation of effective treatment control and vaccine development strategies which may eradicate babesiosis.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Molecular Biology and Biotechnology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. M. Yasir Zahoor
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Ali Ahmed Sheikh
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2017-01-26 2642-T 2642-T Thesis


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