Study On The Diversity And Antibiotic Resistance Profile Of Bacteria Isolated From Respiratory Tract Infected Dogs And Human (Record no. 11035)

000 -LEADER
fixed length control field 06821nam a22002057a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20170425101929.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 170425b2016 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2696-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Misbah Sajjad (2010-VA-299)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Arfan Ahmad
245 ## - TITLE STATEMENT
Title Study On The Diversity And Antibiotic Resistance Profile Of Bacteria Isolated From Respiratory Tract Infected Dogs And Human
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2016.
300 ## - PHYSICAL DESCRIPTION
Number of Pages 73p.;
502 ## - DISSERTATION NOTE
Dissertation note In developing countries, respiratory tract infections are the major reason of the prevalence of disease and death in the patients who are seriously sick. Respiratory problems in human and dogs are common in Pakistan. Broad spectrum antibiotics are being used to treat the infections but duration of illness is increasing day by day. Present study was therefore designed to explore the diversity of bacteria causing respiratory tract infections in dogs and human and antibiotic resistance pattern of bacterial isolates
Total 60 Samples (sputum, nasal discharge, tracheal swab etc) were collected and evaluated. Thirty (n=30) samples were taken from human and dog each. Among 30 samples of human, 10 were taken from infected males and females each. 5 samples from healthy males and females each were also included. Similarly among 30 samples of dogs, 10 were taken from infected dogs and bitches each along with 5 samples from healthy dogs and bitches each.
Samples collected from each gender of human and dog were cultured on the MacConkey, Blood and Chocolate agar plates for the isolation of pathogens. Isolated were confirmation by biochemical tests followed by determination of their antibiotic susceptibility pattern by using Kirby Baur disk diffusion method.
From male samples, Staph spp were isolated from all the samples (100%) followed by E.coli (70%), and Salmonella spp, Haemophilus spp each (30%). Whereas from females, Staph aureus were also isolated from 100% samples followed by Salmonella spp (60%), E.coli (40%), and Haemophilus spp (0%)
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From healthy males, isolation of Staph aureus was confirmed in (100%) samples followed by Salmonella spp (80%), E.coli (20%), and Haemophilus spp (0%). Whereas from females, Staph aureus was also confirmed in (100%) samples followed by E.coli (80%), Salmonella spp (20%) and Haemophilus spp (0%).
From dog samples, Staph aureus was isolated from (100%) samples followed by E.coli (60%), Salmonella spp (40%) and Haemophilus spp (20%). Whereas from bitches, Staph aureus was also isolated from (100%) samples followed by Salmonella spp (60%), E.coli (50%), and Haemophilus spp (0%)
From the samples of healthy dogs, Isolation of Staph aureus was confirmed in (100%) samples followed by E.coli (80%), Salmonella spp (20%) and Haemophilus spp (0%). Whereas from bitches, Staph aureus was also isolated from (100%) samples followed by Salmonella spp (80%), E.coli (20%), and Haemophilus spp (0%)
Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from infected dogs showed 100% resistance to Amoxicillin, Tylosine and Lincomycin and 100% sensitivity to Doxycycline and Oxytetracycline. E.coli showed 100% resistance to Amoxicillin and Lincomycin and 80.8% sensitivity to Ciprofloxacin. Staph spp showed 100% resistance to Tylosine and Lincomycin and Haemophilus spp showed 100% resistance to Ciprofloxacin and Doxycycline
Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from healthy dogs showed 100% resistance to Tylosine and Lincomycin. E.coli showed 100% resistance to Tylosine and Lincomycin and 75.8% sensitivity to Ciprofloxacin. Staph spp showed 100% resistance to Tylosine and Lincomycin and 75% sensitivity to Doxycycline. There was no
Summary
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positivity observed for Haemophilus spp in all samples of healthy dogs, so no sensitivity was performed
Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from infected bitches showed 100% resistance to Tylosine and Lincomycin and 70% sensitivity to Doxycycline. E.coli showed 100% resistance to Tylosine and Lincomycin. Staph spp showed 100% resistance to Tylosine and Lincomycin and 70% sensitivity to Doxycycline. There was no positivity observed for Haemophilus spp in all samples of infected bitches, so no sensitivity was performed
Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from healthy bitches showed 100% resistance to Tylosine and Lincomycin and 90% sensitivity to Doxycycline. E.coli showed 100% resistance to Amoxicillin, Tylosine and Lincomycin. Staph spp showed 100% resistance to Tylosine and Lincomycin and 70% sensitivity to Doxycycline. There was no positivity observed for Haemophilus spp in all samples of healthy bitches, so no sensitivity was performed
Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from infected males showed 80% resistance to Amoxicillin and Tylosine. E.coli showed 100% resistance to Tylosine and Lincomycin. Staph spp showed 100% resistance to Lincomycin and 100% sensitivity to Doxycycline. Haemophilus spp showed 100% sensitivity to Doxycycline
Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from healthy males showed 100% resistance to Lincomycin. E.coli showed 100% resistance to Tylosine and Lincomycin. Staph spp showed 100% resistance to Tylosine and Lincomycin. There was no
Summary
70
positivity observed for Haemophilus spp in all samples of healthy males, so no sensitivity was performed
Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from infected females showed 100% resistance to Amoxicillin, Tylosine and Lincomycin. E.coli showed 100% resistance to Amoxicillin and Lincomycin. Staph spp showed 100% resistance to Lincomycin. There was no positivity observed for Haemophilus spp in all samples of infected females, so no sensitivity was performed
Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from healthy females showed 100% resistance to Lincomycin. E.coli showed 100% resistance to Amoxicillin and Lincomycin. Staph spp showed 100% resistance to Tylosine and Lincomycin. There was no positivity observed for Haemophilus spp in all samples of healthy females, so no sensitivity was performed
Conclusion
Our study showed that samples of respiratory tract collected from human and dog showed involvement of bacteria such as Salmonella spp, E. coli and Staph aureus and Haemophilus spp. Most of strains were multidrug resistant. Ciprofloxacin, Doxycycline and Oxytetracycline were effective to treat respiratory infections in human and dogs.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Microbiology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Aamir Ghafoor
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr.Muhammad Hassan Mushtaq
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2017-04-25 2696-T 2696-T Thesis


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