Prevalence Of Cpb2 In Clostridium Perfringens Isolated From Livestock In Punjab Pakistan (Record no. 11090)

000 -LEADER
fixed length control field 02513nam a22002057a 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20170523105102.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 170523b2016 xxu||||| |||| 00| 0 eng d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 2209-T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Iqra Baig (2009-VA-228)
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Aamir Ghafoor
245 ## - TITLE STATEMENT
Title Prevalence Of Cpb2 In Clostridium Perfringens Isolated From Livestock In Punjab Pakistan
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2016.
300 ## - PHYSICAL DESCRIPTION
Number of Pages 54p.;
502 ## - DISSERTATION NOTE
Dissertation note The current study designed for the microbiological and molecular characterization of
C. perfringens. C. perfringens is Gram positive rod shape, spore forming bacteria and these are free living bacteria. It is suggested by different studies that the C. perfringens has ability to producing different disease in intestine wall of bovines.
Mostly C. perfringens has ability of producing different variety of toxins and enzymes that are responsible for severe myo-necrotic lesions. C. perfringens is divided into five groups (A-E) on the basis of ability for producing the major toxins (alpha, beta, epsilon and iota). A total of 100 fecal samples cattle (n=50) and buffalo (n=50) were collected and analyzed to determine the prevalence of C. perfringens contamination. The samples were enriched in Fluid Thioglycollate Medium (FTM), purified on Reinforced Clostridium Medium (RCM) agar and were identified by their culture characters, morphology and biochemical profile. For confirmation of C. perfringens biochemical test, hemolysis on blood agar, lecithinase activity, gelatine liquefaction test and nitrate reduction test were performed. C. perfringens was successfully isolated from 17 out of 100 samples with an overall positivity ratio of 17 percent. Seven out of 50 were positive for C. perfringens in cattle while this was 10 in buffalo showing slightly higher percentage of C. perfringens in buffalo. Confirmed C. perfringens isolates through biochemical test were subject for DNA extraction by boiling method and kit method both. Isolates of C. perfringens confirmed through biochemical testing were subject for PCR to confirm cpb2 toxin gene. Zero prevalence was found from PCR results.
CHAPTER 6
SUMMARY
Summary
47
Conclusion:
The study reveals that there is high prevalence of C. perfringens among buffalo than cattle. C. perfringens is prevalent in our local animals but beta2 toxin gene not found in the C. perfringens.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Microbiology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Jawad Nazir
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Waseem Shahzad
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2017-05-23 2209-T 2209-T Thesis


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