In Vitro Biological Control Of Avian Pathogenic Escherichia Coli (Record no. 13502)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03626nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20170919085007.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 170918b2017 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2829-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Aleena kokab (2011-VA-418) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Ali Ahmad Sheikh |
| 245 ## - TITLE STATEMENT | |
| Title | In Vitro Biological Control Of Avian Pathogenic Escherichia Coli |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2017. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 78P.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Avian pathogenic E. coli (APEC) has meticulous virulence properties making it a potential pathogen causing insidious infections in poultry, termed as colibacillosis either as primary pathogen or as a secondary pathogen. All over the world, there are major economic losses in poultry industry due to this disease. To prevent APEC infection, strategies include improving hygienic methods, vaccination, antibiotic treatment and introduction of novel immunopotentiators but all these measures had limited success. Moreover, because of extensive use of antibiotics for treatment purposes, antibiotic resistance is major growing concern. Bacteriophage therapy is promising new alternative to antibiotics. There are many benefits of phages over antibiotics and are now used in research for the treatment of enteric and respiratory problems in poultry and are proved to be effective in reducing E.coli infection. In present study bacteriophages was used to minimize and control the number of E.coli causing colibacillosis in poultry. For this, E.coli (n = 10) from liver of infected commercial poultry birds and E.coli (n = 5) from intestines of apparently healthy birds was isolated and characterized using biochemical tests. Final confirmation of E.coli isolates was done by PCR. To differentiate between pathogenic and non-pathogenic E.coli, Congo Red Dye binding test (CR test) was performed. Antibiotic resistance profiling of the isolates was also determined against 10 commonly used antibiotics through Kirby-Bauer disk diffusion method. For the isolation of bacteriophages, sewage or poultry sludge sample was processed according to literature method and purified bacteriophages (n = 5) were Summary 63 assessed for their stability at various temperature and pH. Bacteriophages were also assessed for their In-Vitro Lytic Activity of against E. coli isolates. Five numbers of lytic bacteriophages were isolated and purified against avian pathogenic E.coli, showing resistance against Ampicillin, Doxycycline, Ciprofloxacin, Neomycin, Trimethoprim-sulphamethoxazole, Nalidixic acid and Ceftriaxone. Isolates were also classified into pathogenic and non-pathogenic on the basis of their ability to bind Congo red dye on Congo red media. Phages were highly lytic against pathogenic isolates while there was no lytic activity against non-pathogenic isolates thus making them unique for in vivo trials. Phages were found to be stable at temperature from 25oC to 56oC with highest stability at 37oC while on the basis of pH, phages were stable between 5-9 pH with highest stability at pH 7. Maximum lytic activity observed was for 12 hours and then there was emergence of resistant bacteria and this problem may be tackled by increasing MOI or by using combinations of phages against resistant bacteria. Present study helped to reveal the effectiveness of bacteriophages against antibiotic resistant E.coli and proved that bacteriophages can be used as promising alternatives to antibiotics for reducing E.coli infection in poultry. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | MICROBIOLOGY |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Masood Rabbani |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Waseem Shehzad |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2017-09-18 | 2829-T | 2829-T | Thesis |