Standardisation Of Indirect Haemagglutination Test For Monitoring Infectious Bursal Disease Virus (Record no. 2277)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02364nam a2200205Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20150917154153.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150525s1997 xx 000 0 und d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 0529,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Sajid Mahmood |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Muhammad Akram Muneer |
| 245 ## - TITLE STATEMENT | |
| Title | Standardisation Of Indirect Haemagglutination Test For Monitoring Infectious Bursal Disease Virus |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 1997 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Indirect haemagglutination (IHA) test was standardized and evaluated to moniter antibodies against infectious bursal disease (IBD). It was observed that oil based vaccine prepared from bursae of fäbricius of infected birds, induced a high level of antibody which were detected by agar gel precipitation test (AGPT). It was recorded Chat tannic acid, glutaraldehyde and chromium chloride had 0.0000781, 0.003906 and 0.0001562 per cent subagglutinating dilutions in normal saline solution (pH 7.2) respectively while 0.000001220, 0.0156 and 0.0025 subagglutinating dilution of the coupling agents were found in phasphate buffered saline (pH 7.2), respectively. Indirect. haemagglutination test is sensitive and specific serological technique to study infectious bursal disease. However, antigen dilution to sensitize erythrocytes, source of erythrocytes, chemical nature of diluent, interaction temperature and time, nature and concentration of coupling agent coated erythrocytes and antiserum against IBD, had influenced the sensitivity of IHA test. Ten percent antigen for sensitizing sheep erythrocytes, incubation temperature of 37°C for 10 minutes for antigen, tannic acid (0.005%) and erythroéyte interaction, freshly prepared sensitized erythrocytes and normal saline solution (pH 7.2) as diluent were found suitable for detecting maximum titre of anti-IBD antibodies through the IHA. Moreover it was observed that the standardized IHA proportionally showed reduction in the titre on dilution of serum. The antibody titre in the IHA was the well having serum dilution, showing resistance to bleed (flow) on tilting the plate for 5 seconds. The final results of antibody titre were achieved within 120 minutes post processing of the samples. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Hajid |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Khushi Muhammad |
| 710 ## - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Faculty of Veterinary Sciences |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-05-27 | 0529,T | 0529,T | Thesis |