Standardization Of Indirect Haemagglutination Test For Surveillance Of Antibodies To Mycoplasma Capri (Record no. 2380)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01938nam a2200205Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20150918150438.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150525s1999 xx 000 0 und d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 0637,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Shaukat Ali |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr.Muhammad Amin Sheikh |
| 245 ## - TITLE STATEMENT | |
| Title | Standardization Of Indirect Haemagglutination Test For Surveillance Of Antibodies To Mycoplasma Capri |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 1999 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | The present study was carried out to standardize indirect haemagglutination test for surveillance of antibodies against Mycoplasma capri. The Mvconlasma mvcoides sub species capri strain PG3, procured from Veterinary Research Institute, Quetta was used as antigen in the test. The strain was processed for its optimal growth in brain heart infusion broth and subsequently developed into an oil based vaccine for raising hyperimmune serum in the rabbit. The titre of the latter was made a base for knowing influence of various factors to be studied. The factors examined included the antigen dilution to sensitize erythrocytes, nature and concentration of coupling agent (Tannic acid, glutaraldehyde), source of erythrocytes, interaction time and temperature. One percent sheep and human group 'O' erythrocytes sensitized with antigeh through tannic acid concentration of 0.5% in phosphate buffer saline at 37oC for 10 minutes gave a titre respectively of 1:64 and 1:16. None of the tannic acid concerntrations used (0.5, 0.05, 0.005%) sensitized poultry erythrocytes with antigen at either time interaction. All the various glutaradehyde concentrations used (0.1, 0.2, 0.25, 0.5%) were unable to sensitize erythrocytes, with antigen, irrespective of the source concerned i.e. sheep, human or poultry sources. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr.Asif Rabbani |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr.Khushi Muhammad |
| 710 ## - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Faculty of Veterinary Sciences |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-05-27 | 0637,T | 0637,T | Thesis |