Effect Of Various Extenders On Semen Characteristics Of Beetal Buck (Capra Hircus) (Record no. 2530)

000 -LEADER
fixed length control field 03283nam a2200205Ia 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20150921135336.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150525s 2003 xx 000 0 und d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 0798,T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Aoun Raza
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Prof. Dr. Muhammad Aleem Bhatti
245 ## - TITLE STATEMENT
Title Effect Of Various Extenders On Semen Characteristics Of Beetal Buck (Capra Hircus)
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2003
502 ## - DISSERTATION NOTE
Dissertation note The artificial use of germ cells of genetcally superior bucks can enhance
the growth potential of goat population to meet the shortage of mutton meat in Pakistan for daily slaughtering, sacrificial events, skin, hair and goat milk too.
Beneficial use of superior germ cells can be made only when there is extension of life of germ cells for long periods, maintenance of motility of spermatozoa and increase in number of doses of ejaculate, for which an extender of choice has to be developed. Semen characteristics of forty ejaculates of bucks were evaluated. Pooled samples of ejaculates having motility estimates of at least 60% were used for evaluation. After washing of seminal plasma with physiological normal saline (20% ringer solution) and centrifugation at l000xg for 10 minutes to remove the sperm toxic factor Lecithinase-A. Pooled semen samples were extended in Tris yolk fructose citric acid (TYFCA), milk yolk (MY) and egg yolk citrate (EYC) extenders. Samples were extended using one
step extension at a ratio of 1:60 in such a way that each milliliter of semen contained 30x106 progressively motile spermatozoa. Finally extended semen samples were placed at 5°C and 37°C for evaluation of motility percentage after every 24 hours interval and livability (hours) and absolute index of livability o spermatozoa, respectively. Mean±S.E. values of ejaculates of bucks for volume, pH, mass motility, individual motility percentage, sperm cell concentration, live and dead percentage, sperm abnormalities was recorded and post-extension motility percentage at 5oC, livability (hours) and absolute index of livability of of' spermatozoa of pooled semen at 37°C was recorded. Significant differences were observed (P<0.01) in post extension motility percentage at 5°C, at all intervals except at 120 hours interval where deference vas non-significant (P>0.05) between milk yolk and egg yolk citrate extenders. Post extension motility percentage at 5°C was highest in TYFCA than MY and EYC e\tenders. Post extension livability (hours) at 37°C was significantly different among three extenders at a level of probability (P<0.05) but non-significantly different among three extenders at a level of' probability (P>0.01). Absolute index of' livability at 37°C shows significant differences (P<0.01) for all extenders under statistical analysis. Livability (hours) and absolute index of' livability was higher in TYFCA than MY and EYC extenders. Based on these results and effect of extenders on semen characteristics of Beetal Buck, Tris- Yolk-Fructose-Citric Acid was developed as an extender of choice for short term preservation of' semen of' Beetal Buck.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Theriogenology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr.Muhammad
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr.Muhammad Ashfaq Ahmad
710 ## - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Veterinary Sciences
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-05-28 0798,T 0798,T Thesis


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