| 000 -LEADER |
|---|
| fixed length control field |
02123nam a2200205Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20150921154127.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
150525s2005 xx 000 0 und d |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
0910,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
|---|
| Personal name |
Syed Amer Mahmood |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
|---|
| Location of meeting |
Prof. Dr. Ijaz Ahmed |
| 245 ## - TITLE STATEMENT |
|---|
| Title |
Effect Of Cold Shock On Frozen-Thawed Spermatozoa Of Buffalo & Cow Bulls |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Year of publication |
2005 |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Effect of cold-shock on frozen-thawed bovine spermatozoa was measured in terms of motility, viability, plasma membrane and acrosome . integrity. Single ejaculates each from seven Nili-Ravi buffalo bulls and 7 Sahiwal cow bulls were processed for freezing. Three straws of 0.5ml (for each parameter) from each bull were thawed and pooled at 3rC before and after cold-shock at 4°C for 2 min. Individual motility was observed by phase contrast microscope before and after cold-shock. Before and after cold-shock, sperm viability, plasma membrane and acrosome integrity were evaluated by supravital stain, hypo osmotic swelling test and normal acrosomal reaction, respectively. Two hundred sperm were counted for each parameter before and after cold-shock. Comparison of before and after cold shock values showed that cold shock had no effect (P>0.05) on motility (64.0±1.88 vs 60.4±2.56), viability (141.1±4.39 vs 124.5±8.03), plasma membrane integrity (83.51±5.82 vs 71.19±4.31) and acrosome integrity (148.3±2.36 vs 141.1 ±2.85) of buffalo bull spermatozoa. Comparison of before and after cold shock values of cow bull spermatozoa indicated that cold shock had significant (P<0.05) effect on motility (59.2±1.05 vs 41.9±.12), viability (140: 14±2.94 vs 90.5±2.73), and plasma membrane integrity (62.3±4.28 vs 47.24±3.71) but no effect on acrosome integrity (147.9±2.21 vs 140.6±2.40). In conclusion, cold shock had significant detrimental effect on cattle bull spermatozoa but no effect on buffalo bull spermatozoa.
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| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Department of Theriogenology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Dr.Azhar |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Dr.Muhammed Amir Saeed |
| 710 ## - ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
Faculty of Veterinary Sciences |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Thesis |
