| 000 -LEADER |
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| fixed length control field |
02062nam a2200205Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20150921154937.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
150525s2005 xx 000 0 und d |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
0926,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
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| Personal name |
Muhammad Babar |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
|---|
| Location of meeting |
Prof.Dr.Muhammad Ashraf |
| 245 ## - TITLE STATEMENT |
|---|
| Title |
Preparation And Evaluation Of Rabbit Anti-Buffalo Immunoglobulin Antibody Peroxidase Conjugate |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Year of publication |
2005 |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Enzyme linked immunosorbent assay (ELISA) is one of the most sensitive rapid and reliable techniques for diagnosis of infectious diseases. For execution of ELISA, antibody-peroxidase conjugate is the fundamental reagent. Turnip peroxidase was purified from turnips, that includes homogenization, inactivation of catalase, ammonium sulphate precipitation and size exclusion chromatography on Sephadex G-25-80. The purified peroxidase had Rz value of 1.7, total protein 0.9 mg/ml and total enzyme activity 36152 units/liter. The buffalo serum Ig-G was fractionated using 40 percent final concentration of ammonium sulphate followed by anion exchange chromatography. The salt fractionated serum globulins (10 ml) was depleted of its Ig-G in less than 25 minutes on DEAE cellulose packed column followed by suitable elution. The Ig-G solution (1.0 gm/dl) was mixed in four times volume of oil base (Liquid paraffin and emulsifiers). Rabbits were primed and boosted (0.25ml/: subcut) with buffalo Ig-G antigen with 21 days interval. The immune serum was harvested on 21 days post-boosting. The serum contained 2048 agar gel precipitation AGP units and 10,000 ELISA units. Rabbit anti buffalo Ig-G was purified with salt precipitation followed by anion exchange chromatography. The peroxidase was linked with the rabbit anti-buffalo Ig-G using the sodium metaperiodate. The conjugate was titrated against buffalo Ig-G and working dilution for execution of ELISA was 1: 2000.
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| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Department of Physiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mr. Shahid Abbas |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Prof. Dr. |
| 710 ## - ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
Faculty of Biosciences |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Thesis |
