Comparison Of Multiplex Pcr & Conventional Methods For The Diagnosis Of Tuber Culosis (TB) in Human, Buffalo & Cattle in Lahore District (Record no. 2677)
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| fixed length control field | 01830nam a2200205Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20150921160407.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150525s2007 xx 000 0 und d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 0954,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Naima Mumtaz |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Prof. Dr. Zafar Iqbal Chaudhary |
| 245 ## - TITLE STATEMENT | |
| Title | Comparison Of Multiplex Pcr & Conventional Methods For The Diagnosis Of Tuber Culosis (TB) in Human, Buffalo & Cattle in Lahore District |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2007 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Tuberculosis, one of the most widespread infectious diseases, is the leading cause of death due to single infectious agent among humans and animals in the world. It is endemic in Pakistan with about 1.5 million people infected, and Pakistan ranks seventh among the 22 high-burden tuberculosis countries worldwide (WHO, 2006). Mycobacterium tuberculosis is the most common cause of human TB, but an unknown proportion of cases are due to Mycobacterium. bovis. The study was conducted in Lahore to compare the multiplex PCR and conventional methods for the diagnosis of tuberculosis caused by M tuberculosis and M bovis in 300 humans' sputum and 1000 bovines' milk samples. Conventional methods included Ziehi Neelsen staining, culture and biochemical tests. For M tuberculosis and M bovis the pncA gene and specie -specific 500 bp fragments were targeted respectively. The sensitivity and specificity of multiplex PCR was found statistically significant in comparison to Ziehl Neelsen staining and culture for the differential diagnosis of TB. Pyrazinamide resistance was found in 15 (34.8%) out of 43 isolates recovered from media inoculated by sputum and milk. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Pathology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Asim Aslam |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Abdul Rarf Shakoori |
| 710 ## - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Faculty of Veterinary Sciences |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-05-29 | 0954,T | 0954,T | Thesis |