| 000 -LEADER |
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| fixed length control field |
01880nam a2200193Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20151008141559.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
150525s2007 xx 000 0 und d |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
0984,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
|---|
| Personal name |
Mubashir Hussain |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
|---|
| Location of meeting |
Prof. Dr. Muhammad Akram Muneer |
| 245 ## - TITLE STATEMENT |
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| Title |
Factors Affecting Hemagglutination Potential Of Avain Influenza Viuruses (H5, H7, H9 Subtypes) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Year of publication |
2007 |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
The objective of this study was to standardize hemagglutination and hemagglutination inhibition tests for AIV H5, H7 and H9 subtypes. These subtypes were propagated in 09-day old chicken embryonated eggs and after 72 hours post incubation the allantoic fluid (AF) was harvested and confirmed by spot agglutination test and by AGPT. While standardizing HA test maximum titers were recorded using 1% RBCs of chicken, human blood group Qe and dog using phosphate buffer saline (PBS) as a diluting agent for washing suspension of erythrocyte and by incubating the micro titer plates at 22c or 37C for 30 minutes or 40 minutes time period. The AIV subtypes eluted rapidly with increase in temperature with maximum elution observed within the time period of 8 hours. The live AIV provided much higher HA titer when compared with the titers obtained from AJV subtypes inactivated with formalin or Binary ethylene imine (BET). The BET was found to have little effect on HA activity as compared to formalin. While standardizing the HI test the best titers were obtained using 4 HA units of AIV antigen as compared to 1 HA and 8 HA units of antigen and by incubating the micro titer plates for 60 minutes period (time given for antigen-antibody reaction before the addition of erythrocytes suspension).
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| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Department of Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Dr. Mansur-ud-Din Ahmad |
| 710 ## - ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
Faculty of Veterinary Sciences |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Thesis |
