A Study On Single Nucleotide Polymorphism Of Chicken Growth Hormone Gene In White Cornish ,Lyall Pur Silver Black ,Aseel and Desi Chicken of Pakistan (Record no. 2725)
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| fixed length control field | 05571nam a2200193Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20150930142418.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150525s2008 xx 000 0 und d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 1003,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Mr.Imran Zahoor |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr.Muhamad Akram |
| 245 #2 - TITLE STATEMENT | |
| Title | A Study On Single Nucleotide Polymorphism Of Chicken Growth Hormone Gene In White Cornish ,Lyall Pur Silver Black ,Aseel and Desi Chicken of Pakistan |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2008 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | The present study was conducted at Molecular Cytogenetics and Genomic Lab, Department of Livestock Production, University of Veterinary and Animal Sciences, Lahore and School of Biological Sciences, Punjab University, Lahore. For this Purpose twelve chickens, three each of four different breeds (White Cornish, Lyall pur Silver Black, Aseel, Desi,) were selected. LSB birds were collected from Agriculture University, Faisalabad. Where as birds of Desi and Aseel chickens were taken from Mianwali. White Comish birds were obtained from Big Bird Poultry breeders, Lahore. The pituitary gland of all the twelve birds of the above mentioned four breeds were taken out to extract the mRNA that was then converted into eDNA by using RT-PCR. Then that cDNA was amplified through PCR reaction. After amplification, the PCR products were loaded on agarose gel. Then these PCR products were cloned into vector in three steps. Firstly purified Gg GH mRNA was ligated into linearized pET22b. Then it was transformed into E. coli DH5CL with pTZ57R/GgGHG. Positive clones containing recombinant plasmid (pTZ57R/GgGHG) were screened by "Blue-White screening". Cells from a single colony for each of the W1 -W4 were resuspended separately in 50µl of sterile distilled water. This mixture was then used as template for PCR and the Gg GHG coding sequence cloned in plasmid DNA was then amplified and product was run on gel to study the result. These cloned products were used for the sequencing of growth hormone gene. Further results were obtained by using the consensus sequences of growth hormone of four breeds of chicken in ClustalW2 and Bioedit software and it was found that three SNPs were detected in Aseel, and one in each of Desi and LSB while comparing with White Comish. Out of three, first, second and third SNP were detected in Aseel at location of 261, 435 and 551 position of nucleotide in the exon portion of growth hormone gene, and C is replaced with G at location 261 and C is replaced with T at position 435 whereas at position 551, T is replaced by A. At these locations codon changes from CTC to CTG and TTC to TTC and TTC to TAC respectively. Only one SNP was detected in Desi and LSB at location 513 where A is replaced with G in both the breeds and codon change from AAA to AAG. To determine the relationship among all these breed (White Cornish, Aseel, Desi and LSB) a phylogenetic analysis was done. The phylogenetic tree was constructed using the presence and absence of restriction site and/or genotypic frequencies in the cGH motif of growth hormone gene. The phylogenetic consensus tree was constructed using the boostrapped data and neighbour-joining method. The Nei's genetic distance (Da) values of Aseel was 0. 00970, while comparing the genetic distances of White Comish with Aseel, it is found that there was a difference of 0 . 00320 and LSB has similar genetic distances and having no genetic difference among themselves. As LSB was evolved by crossing four different breeds Desi and White Leg Horn for female line and White Cornish and New Hampshire for male line so LSB is placed in-between Desi and White Comish as it has the 25% blood of Desi and 25% blood of White Cornish. Where as Aseel is different that's why it is placed far distant from three other breeds but as Aseel and Desi and LSB also are breeds of Asian origin and they were originated from red jungle fowl that's why Aseel, Desi and LSB (a cross of Desi with other three breeds; mentioned above) are placed more close to each other than White Cornish that is the breed of English class. In the present study, the Amino Acid compositions of the growth hormone of four different breeds of chicken were also studied and it was found that there although there were three mutations in Aseel but out of these three, two showed silent mutation and only one mutation was active. This active mutation cause change in the amino acid composition of the chicken growth hormone of these four breeds of poultry and in this study Phenylalanine is changed into Tyrosine at the location of 184t11 amino acid out of 191 amino acids (full length chicken growth hormone). But one mutation that was found in each of Desi and LSB in their comparison with White Comish were silent as the new sequences does not cause any change in amino acid sequence of growth hormone gene of Desi and LSB. CONCLUSION: In the present study, three SNPs were detected in the Exon region of the mRNA and one amino acid Phenylalanine is changed to Tyrosine, this amino acid change is in the conserved domain so it can be used as a marker for the further breeding program. RECOMMENDATIONS Present study laid the foundation of molecular techniques in the poultry but still 1. There is need of comprehensive study in which atleast 25 bird should be used in each strain. 2. Along with DNA study, there is need to study the phenotypic characters by rearing these strain upto three generations. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Poultry Production |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr.Abdul Waheed Sahota |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-05-27 | 1003,T | 1003,T | Thesis |