Molecular Detection And Speciation Of The Canme Piropiasm (Record no. 2778)

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fixed length control field 03310nam a2200217Ia 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20170802142511.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150525s2008 xx 000 0 und d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 1061,T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Isma Nazli Bashir
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Prof. Dr.Zrafar Iqbal Ch.
245 ## - TITLE STATEMENT
Title Molecular Detection And Speciation Of The Canme Piropiasm
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2008
502 ## - DISSERTATION NOTE
Dissertation note An epidemiological study of babesiosis in dogs was conducted at Pet center, University of Veterinary and Animal Sciences, Lahore, for one year and information on age, sex and breed was gathered. It was found that from a total number of 6204, dogs up to two years of age were more susceptible than other age groups (2-4, 4-6 and above 6 years).The data regarding genders revealed that males were more prone to the disease than female dogs. As far as the breeds were concerned, crossbred dogs were more susceptible followed by Pointers, Alsatians, German shepherds and Bull terriors.Hot and humid months (June to September) have greater impact on the occurrence of disease. The study regarding identification of ticks revealed that Rhiphicephalus sanguinus is the predominant vector of the disease in Pakistan.
Molecular studies were conducted to characterize and identify the species responsible for canine babesiosis in Pakistan. In this regard, a nested polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLP) technique was employed on different specimens (Blood, Body tissues and Ticks). For this purpose blood samples were collected from twenty four chronically infected dogs and applied on the Flinders Technologies Associates (FTA) cards for transportation to Australia. Different body tissues (Liver, Spleen, Kidney, Intestine, Bone marrow and Pancreas) were procured after euthanizing the two dogs and DNA was extracted, for further studies. Similarly, the eighty eight ticks were also collected from the infested dogs in the 70% ethanol for transportation to Australia. A nested PCR-RFLP assay was used for the detection and differentiation of Piroplasm species on the basis of the 1 8S ribosomal RNA gene. The assay potentially amplified and identified Babesia gibsoni as the main canine piroplasm. Similar assays on the DNA extracted from body tissues and ticks revealed Babesia gibsoni as the main piroplasm. The PCR was found to have a high detection limit (equivalent to i0 dilution), when using the DNA extracted from blood applied to FTA cards, body tissues and ticks. A new technique was developed for extraction of DNA from FTA cards and tick, in this technique, instead of using the FTA specified punching machine, we used scalpel blades, and so the rest of the chemicals used are'generally and easily available. The same protocol was used for extraction of DNA from ticks, only chemicals used in different quantities with different spinning times. Both of which, resulted in cost reduction, less effort and speedy DNA extraction. The technique reported here has the potential to be standardized for routine DNA extractions from FTA cards and ticks.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Parasitology
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Topical Term Phd. Theses
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr.Peter J.Irwin
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Prof. Dr. Azhar Maqbool
710 ## - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Veterinary Sciences
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-05-27 1061,T 1061,T Thesis


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