Use Of Antioxidate Containing Extender To Improve Post Thaw Quality Of Canine Semen (Record no. 2903)

000 -LEADER
fixed length control field 02343nam a2200181Ia 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20151001144511.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150525s2010 xx 000 0 und d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 1184,T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Zia Ullah
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Prof. Dr. Ijaz Ahmad
245 ## - TITLE STATEMENT
Title Use Of Antioxidate Containing Extender To Improve Post Thaw Quality Of Canine Semen
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2010
502 ## - DISSERTATION NOTE
Dissertation note plasma membrane integrity, acrosomal integrity and viability of spermatozoa) using the Labrador retriever dog frozen semen.
Dog is a unique animal in terms of its semen ejaculation which is in fractions with a highly variable sperm concentration in different fractions as compared to other mammalian species like bull, boar, ram, human which have their ejaculate as a single fraction. In this study, BHT was found to improve post thaw motility, sperm viability, plasma membrane integrity and the acrosomal integrity at an inclusion level of 1.0mM in the extended semen. However, the higher concentrations than this were found to have detrimental effects on the parameters mentioned above. The reasons for these detrimental effects could be the change in osmolarity of the semen that varies in different species. The ROS production is associated with normal physiological functioning of the spermatozoa and when a semen sample is diluted the endogenous antioxidant mechanism of the semen is compromised and addition of exogenous antioxidant is needed to equilibrate. Therefore the addition of BHT at an inclusion level 1.0mM in study was found optimum and higher concentrations of this had detrimental effect on the spermatozoa, which could be due to two reasons as explained by Ijaz et al., 2009, i.e., firstly higher concentration of BHT reduce the physiological functions of the spermatozoa by extremely reducing the ROS which are needed to a certain level for physiological function of the spermatozoa and secondly it increases the fluidity of the plasma membrane above the desired levels thus making it prone to more damages.
In conclusion, BHT was found useful for dog spermatozoa at inclusion level 1.0mM, however, further studies are required to investigate the fertility of the canine semen having inclusion levels of BHT.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Theriogenology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Prof. Dr. Muhammad Aleem
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-05-28 1184,T 1184,T Thesis


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