Linkage Analysis Of Myp12 And Myp14 In Families From Lahore (Record no. 2935)

000 -LEADER
fixed length control field 02451nam a2200193Ia 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20151001145808.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150525s2010 xx 000 0 und d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 1216,T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Maryam Zahra
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Prof.Dr.Masroor Elahi Babar
245 ## - TITLE STATEMENT
Title Linkage Analysis Of Myp12 And Myp14 In Families From Lahore
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2010
502 ## - DISSERTATION NOTE
Dissertation note Myopia is one of the most common refractive errors of the eye worldwide that can effect clarity of vision, limit occupational choices and contribute to increased risk to vision threatening conditions. Six families of different casts were enrolled from Lahore (Punjab). Total of six autosomal dominant families were screened for linkage to the known nonsyndromic autosomal dominant and QTL myopia locus, MYP12 and MYP14 respectively. 5mL blood sample were collected aseptically in a 5Oml falcon tubes containing EDTA. DNA extraction was done by inorganic method. Three markers for each locus were selected from literature and redesigned by using 'primer 3' software. These markers were optimized for their annealing temperature and specific concentration of PCR ingredients by gradient PCR. After that all of the markers were amplified separately on genomic DNA samples of each family. PCR products of each of the marker were run on 1.2 % agarose gel along with 50 base pair ladder to visualize the bands of amplified products at 110 volts for 30 minutes. Linkage analyses were carried out by genotyping through PAGE unit of Major Science, model no. MV-2ODSYS. PCR products were run on Polyacrylamide Gel Electrophoresis (PAGE) to examine amplified bands of microsatellites. A standard 5Obp DNA ladder was run along with the sample PCR products as a reference. By reading the alleles appeared on gel haplotypes were constructed for each member of these families. The overall results of this study did not show evidence for linkage of myopia in thee families to the selected loci MYP12 and MYP14 on chromosomes 2 and 1 respectively. It might be possible any other identified locus or any new locus involved in this population of Pakistan. The findings represented here do not represent the conclusion of this study but do provide ongoing data for further investigation into the exact gentic causes of mypia in Pakistan.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Institute of Biochemistry & Biotechnology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr. Ali Raza Awan
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Prof. Dr.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-05-28 1216,T 1216,T Thesis


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