| 000 -LEADER |
|---|
| fixed length control field |
02432nam a2200193Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20151005125803.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
150525s2011 xx 000 0 und d |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
1249,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
|---|
| Personal name |
Rana Adnan Ali |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
|---|
| Location of meeting |
Prof.Dr.Muhammad Ashraf |
| 245 ## - TITLE STATEMENT |
|---|
| Title |
Chemical Equivalence Of Different Brands Of Amoxicillin Trihydrate And Its Minimum Inhibitory Concentration |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Year of publication |
2011 |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
This project was designed to study the chemical equivalence of different brands of amoxicillin trihydrate (long acting and short acting) approved by the ministry of health and available in the market for veterinary use. Amoxicillin was measured by HPLC method developed and standardized in the laboratory. Limit of detection (LOD) and limit of quantification (LOQ) of the amoxicillin trihydrate was determined. Solutions of different concentrations were prepared from amoxicillin trihydrate reference standard for the determination of LOD. and were protected from light and stored at 2-8 oC until used. The LOD calculated by us was 0.100 (µg / ml) and LOQ was 0.5 (µg / ml). Correlation Coefficient should be ? 0.99 and the result obtained by the data was 0.99984050. Chemical equivalence of all brands was determined by using HPLC systems (Shimadzu & Agilent). Concentrations for reference standard (50, 25 and 10 ?g /ml ) and for each brand (Alomox LA, Amovet LA, Farmox LA, Novamox LA, Trioxyl LA, Amoxi-vet, Colimox, and Colimoxin) were used. All the results obtained showed that maximum percentage of assay obtained among long acting was of the brand Farmox LA (101 %) and in case of short acting was of Amoxi-vet (101%). Minimum percentage of assay among long acting was of brand Amovet LA (92 %) and in case of short acting was of Colimox (96%). MIC of amoxicillin against E.coli and Staphylococcus was determined by micro broth dilution test. According to our results 73.33 % E.coli were susceptible and 26.67% were resistant to the amoxicillin trihydrate. Our results showed that 86.67% Staphylococcus were susceptible and 13.33% were resistant to Amoxicillin Trihydrate (Reference Standard). It showed that this antibiotic is still very effective against the diseases produced by the Escherichia.coli and Staphylococcus aureus.
|
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Department of Pharmaoclogy & Toxicology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Dr aftab Ahmad |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Dr.Muhammad Adil Rasheed |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Thesis |
