Sequence Analysis Of Shiga Toxin 1 And Shiga Toxin 2 Genes Of Escherichia Coli O157: H7 Isikates From Lahore (Record no. 3088)
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| fixed length control field | 02450nam a2200181Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20151005151135.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150525s2011 xx 000 0 und d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 1375,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Saqib Hussain |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Mr. Tanveer Hussain |
| 245 ## - TITLE STATEMENT | |
| Title | Sequence Analysis Of Shiga Toxin 1 And Shiga Toxin 2 Genes Of Escherichia Coli O157: H7 Isikates From Lahore |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2011 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Escherichia coli is normal inhabitant of all the animals and human beings. The Sorbitol non fermenting E. coli strains were detected in milk, beef and fecal samples collected from different areas of Lahore. White colored colonies of each positive sample on Sorbitol MacConkey's Agar (SMA) contained gram negative, rods with round ends non spore former Escherichia coli. Each of the isolates was sorbitol non fermenter, lactose fermenter, indole positive, Methyl Red positive, Voges Prauskaur negative and citrate negative. Each of the isolate was further characterized using polymerase chain reaction (PCR) for the presence of shiga toxin 1 and shiga toxin 2 genes. Bacterial DNA was extracted easily by boiling method when the isolates were grown on Sorbitol MacConkey's agar at 37°C for 12-24 hours. The DNA was recovered when the culture was boiled for 5-10 minutes. The isolated DNA when amplified using Stxland Stx2 specific primers showed that 68.5 percent samples were positive for Stxl and 54.2 percent for Stx2. The stx 1 and stx2 PCR products were subjected to sequencing. The resulted sequences when aligned with the reference sequence through Basic local alignment tool it showed that the shiga toxin 1 and shiga toxin 2 gene sequences are conserved and showed high similarity in their nucleotide structure. Despite of having high similarity in their nucleotide structure some haplotypes were also obtained showing single nucleotide polymorphism. Phylogenetic analysis made among local isolates and also with reported sequences from all over the world by using bioinformatics software to see the genetic similarities and difference between them. The data produced showed some highly conserved sequences and SNPs as well that will be quite useful for further applications in diagnostics and biotechnology applications in future. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Institute of Biochemistry & Biotechnology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr.Masroor Elahi Babar |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-05-29 | 1375,T | 1375,T | Thesis |