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| fixed length control field |
03246nam a2200193Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20151005151245.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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150525s2011xx 000 0 und d |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
1378,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
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| Personal name |
Muhammad Mudassar Manzoor |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
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| Location of meeting |
Mr. Taneer Hussain |
| 245 ## - TITLE STATEMENT |
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| Title |
Genetic Diversity And Differentiation Of Domestic Buffalo Of Pakistan Through Sky And Zfy Genes Of Y Chromosome |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Year of publication |
2011 |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Livestock sector plays a vital role in the economy of Pakistan. Main contribution of milk comes from buffaloes and cows. Water buffalo (Bubalusbubalis) is one of the major elements of livestock in the country and possess great importance for economy in the form of milk and meat productions. Nili, Ravi,Nili-Ravi, Kundi and Azakheli are major breeds of water buffalo to be found in different areas of Pakistan. Conventional classification of breeds was based on phenotypic traits. In some cases, recent genetic studies have found differences in the structure proposed. In buffalo ,one has to bear in mind that morphological changes were not the result of adaptation to the environment, but have a social significance thus may not be indicative of the genetic relationship. In recent years Y chromosomal genes have proved to be very useful for the determination of genetic relationship among population. Comparative studies have highlighted the advantages of the SRY and ZFY genes of Y chromosome.These genes have been considered as competent and powerful tool for the purpose of breed characterization and species identification of buffaloes. In livestock sector, water buffalo (Bubalusbubalis) has shown great prospective in numerous Asian countries including Pakistan. Unfortunately, there is lack of genetic data of different buffalo breeds like Nili-ravi and Kundi which needs to be established for their genetic identification.Blood samples from true representative animals of each of the two buffalo breeds (Nili-Ravi and Kundi ) were collected from different Government livestock farms and their respective home tractsin Punjab and Sindh respectively. DNA was extracted by inorganic method and amplification of the SRY and ZFY(exon 5) genes of Y chromosome was done with especially designed primers using Primer3 software in Molecular Biology and Genomics Laboratory at Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore.Specific primers are designed for these genes amplification. Then primers were optimized for successful amplification with minimum reagent concentration. PCR was performed for amplification of SRY and ZFY(exon 5)genes on each sample. Sequencing was conducted on amplicons to find out the different single nucleotide polymorphism (SNP) to make haplotypes with the help of bioinformatics software like Blast 2sequence and Neighbour Joining phylogenetic tree was constructed by using MEGA version 5 (Tamuraet al. 2011). The results obtained from this study now can contribute to the establishment of routine DNA typing service to the advantages of the buffalo in livestock industry.
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| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
Institute of Biochemistry & Biotechnology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Mr. Muhammad Asif |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Prof. Dr. Abu |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Thesis |
