Identification Of Single Nucleotide Polymorphisms In Olri Gene And Its Association With Milk Composition In Cattle Breeds in Pakistan (Record no. 3105)
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| fixed length control field | 03509nam a2200193Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20151005152526.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150525s2011 xx 000 0 und d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 1396,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Nusrat Majeed |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Prof.Dr.Masroor Elahi Babar |
| 245 ## - TITLE STATEMENT | |
| Title | Identification Of Single Nucleotide Polymorphisms In Olri Gene And Its Association With Milk Composition In Cattle Breeds in Pakistan |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2011 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Pakistan being agriculture based country has a great potential in livestock sector. it plays an important role in the economy of the country. Milk is a balanced diet because it contains all the essential nutrients like carbohydrates; fats, proteins, vitamins, minerals and enzymes required for health. In these milk nutrients fat is second most important component of milk. Primary component of milk fat is triglycerides (triacylglycerols or TAG) a typical storage form of lipids. OLRI is the major protein that binds, internalizes and degrades oxidized low density lipoprotein. Ol.Rl as a protein important for oLDL metabol ism may contribute to these effects. Oxidized fat inhibits the expression of lipoprotein lipase and fatty acid transporter genes that causes a reduced uptake of fatty acids into mammary glands. As a result the concentration of triacylglycerols in milk is reduced. OLRl as a protein important for oLOL metabolism may contribute to these effects. OLR J identified as a functional and positional candidate gene product for milk fat percentage and milk fat yield. Polymorphism in Ol.Rl gene increases the milk fat percentage. If polymorphism in Ol.Rl gene is found in Pakistani Sahiwal and Ohanni cattle breeds these were help to screen the animals at younger age and also be used to characterize the different cattle breeds as a milk fat production marker. Unrelated animals were selected for this study. Blood samples were collected from different Go\'1. livestock farms/experimental stations. DNA was extracted by organic method. Primers were designed by using Primer3 software. After DNA amplification by polymerase chain reaction. peR product was sequenced. Sequencing results of the full length OLR I gene were analyzed by alignment of sequences with the help of Blast2sequence software. Novel S Ps were identified. Total of twenty one SNPs were identified in the samples of all breeds that were confirmed at population level by sequencing of each sample. Then total twenty one SNPs were found in all breeds. Most of SNPs were found in intronic regions. In Sahiwal only 5 SNPs are exonic and one of them is common in both Sahiwal and Red Sindhi breeds. Out of total five exonic SNPs, only two were found synonymous and rest of the three exonic SNPs were found to be non synonymous. while all other SNPs are intronic in nature. An intronic SNP is also common between Sahiwal and Dhanni breed. In Red Sindhi a SNP was observed at 3'UTR. Results were analyzed by. using the statistical software POPgene32. The mean value of expected heterozygosity that is 0.3595 is greater than the mean of observed heterozygosity that is 0.0300 that shows low variabil ity in breeds. The mean value for Shannon's Information index (1*) is 0.5421. The aim of research is to identify gene that underlie the genetic variation in bovine milk. fat composition. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Institute of Biochemistry & Biotechnology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Ali Raza Awan |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Abu |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-05-29 | 1396,T | 1396,T | Thesis |