| 000 -LEADER |
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| fixed length control field |
02278nam a2200193Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20151005152827.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
150525s2011xx 000 0 und d |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
1400,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
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| Personal name |
Sehrish Basheer |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
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| Location of meeting |
Dr. Asif Nadeem |
| 245 ## - TITLE STATEMENT |
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| Title |
Molecular Approach For Sex Determination In Avian Species |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Year of publication |
2011 |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
To tell the difference between male and female birds takes keen observation. While not all bird species have easily visible gender differences. Almost all birds' species are sexually monomorphic so it is difficult to distinguish between male and female characters. There are some techniques for bird's sex identification. Surgical Sexing, in this an endoscope is passed into the body cavity and ovaries or testis can be observed. It is available for all types of birds. Surgical sexing is oldest and quickest method. But it cause stress to the bird and expensive technique. Fecal steroid analysis is another technique in which stool sample is analyzed for reproductive hormone presence. In this technique bird should be sexually mature for analysis. Bload Sexing is used to determine the male or female bird. This can be done at any age. Feather exing is very useful and economical method. Retrieve a feather pulp from growing feather and analyze the presence of male and female chromosome. Two chromosomes Z and Ware present in birds. ZZ in male and ZW in female. The method used in this study was based on the chromosome differences. Nine different species of birds which includes green parrot, budgerigar, .pigeon, quail, sparrow, chicken, peacock, duck and pheasants were analyzed and their sex is determined by molecular methods. DNA will be extracted from feathers and blood. The intronic egion of CHD 1- Wand CHD l-Z gene will be amplified by sex specific primer. PCR products were screened by agarose gel electrophoresis. The PCR products were show double and single bands on the agarose gel. The double bands indicate female bird because of ZW chromosome is present in females and single band indicates the male bird because of ZZ chromosomes in males.
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| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
Institute of Biochemistry & Biotechnology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dr. Muhammad Wasim |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Madam |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Thesis |
