Serum Biochemical Metabolites, Electrolytes And Minerals Profiling In Heat Stressed Donkeys (Equus Asinus) (Record no. 3143)

000 -LEADER
fixed length control field 03274nam a2200193Ia 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20151006124838.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150525s2012xx 000 0 und d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 1437,T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Naz Fatima
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Muhammad Shahbaz Yousaf
245 ## - TITLE STATEMENT
Title Serum Biochemical Metabolites, Electrolytes And Minerals Profiling In Heat Stressed Donkeys (Equus Asinus)
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2012
502 ## - DISSERTATION NOTE
Dissertation note Background
In developing countries, equines are used as a companion animal, for transportation, carrying goods, pulling carts and to ride. Heat stress (HS) is the major problem of tropical and subtropical region that adversely affects the performance of livestock by altering the physiological indices.
Objective
The current study is designed to report the dynamics of different physiological indices including serum electrolytes, minerals, biochemical metabolites, hepatic enzymes and thyroid hormones in heat stressed (HSd) donkeys (Equus asinus).
Materials and Methods
A total of 20 donkeys were involved (10 healthy and 10 clinically diagnosed as HSd), irrespective of their gender and age, in the current study. The health of the donkeys was evaluated by observing vital signs including body temperature, respiratory rate, heart rate and hematocrit. Blood was collected from each animal during summer season in August and serum was harvested by centrifugation, of clotted blood, at 4,000 rpm for 20 minutes. Serum samples were stored at -20°C for analysis. Serum electrolytes and minerals were determined using flame photometry and atomic absorption spectrophotometer, respectively, after wet digestion of serum. Serum biochemical metabolites and hepatic enzymes were evaluated using commercial kits, spectrophotometrically. Serum thyroid hormones concentrations were determined using commercial ELISA kits. Normal distribution of the data was confirmed by Shapiro-Wilk test. Data was analyzed using Independent Student's t-test by employing Statistical Package for Social Science (SPSS). Data was presented as means ± SE. Differences were considered significant at P < 0.05.
Results
Results revealed significantly higher heart rate, respiratory rate, rectal temperature and hematocrit in heat stressed donkeys. Results revealed that serum sodium and potassium concentrations were significantly lower and serum calcium, magnesium and iron concentrations were significantly higher in heat stressed donkeys. However, there was no significant difference in Na/K ratio and serum chloride in HSd donkeys. Results revealed significantly higher serum total proteins, albumin, globulins concentrations, A/G ratio, LDL-cholesterol levels, T3, T4, AST, ALT, and ALP activity and homocysteine concentrations in heat stressed donkeys. However, serum HDL-Cholesterol and triglycerides levels were significantly lower. No significant difference in concentrations of serum glucose and cholesterol was observed.
Conclusion
Heat stress decreases electrolytes concentration and increases homocysteine and LDL-cholesterol levels that may account for increased risk of cardiovascular manifestations.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Physiology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Prof. Dr. Habib-ur-Rehman
710 ## - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Biosciences
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-05-29 1437,T 1437,T Thesis


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