| 000 -LEADER |
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| fixed length control field |
02344nam a2200181Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20151006130549.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
150525s2012 xx 000 0 und d |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
1476,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
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| Personal name |
Nausheen Saba Khalid |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
|---|
| Location of meeting |
Dr. Muhammad Ovais Omer |
| 245 ## - TITLE STATEMENT |
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| Title |
Effects Of Lycopersicon Esculentum And Citrus Limon Juice On Angiogenesis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Year of publication |
2012 |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Lycopersiconesculentumand Citrus limonumare largely known for their anti cancer activity. Various investigations using various assays have been done regarding anti cancer activity of Lycopersiconesculentumand Citrus limonum. This study wasplanned to estimate the effects of LycopersiconesculentumandCitrus limonumjuice on angiogenesis using the chick chorioallantoic membrane model. LycopersiconesculentumandCitrus limonum wascollected, sliced to squeeze juice, centrifuged and then supernatant was collected. Different dilutions were prepared using distilled water. PH was adjusted in range of 6-7 and filtered using disposable syringe filter. Fertilized eggs wereobtained from local hatchery and sprayed with 70% alcohol then divided in to six groups containing five eggs in each group one group act as control. The eggs were incubated at 37C° and at60_65% humidity. On 4th day postincubationwindows were made in all eggs under strict aseptic conditions, albumin was aspirated (approximately 4 -5 ml) with a disposable syringe, sealed with sterilized adhesive tapes and kept in incubator for 24 hours.After 24 hours windows were opened, 200 ?l of each dilution waspoured on CAM with disposable syringe, sealed again with sterilized adhesive tapes and again kept in incubator till 6th day. After 24 hours pattern of development of new blood vessels were assessed in CAM by taking images with the help of digital camera.Decrease in length and diameter of primary, secondary and tertiary blood vessels were seen in a concentration dependent way. All images were quantified by using scan probing image processing software (IBM- Denmark). 3D surface roughness parameters were calculated that evidently showed antiangiogenic nature of tomato and lemon juice. All the calculated data was subjected to statistical analysis.
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| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Department of Pharmaoclogy & Toxicology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Prof. Dr. Muhammad Ashraf |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Thesis |
