000 -LEADER |
fixed length control field |
02316nam a2200193Ia 4500 |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20151006130625.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
150525s2012 xx 000 0 und d |
041 ## - LANGUAGE CODE |
Language code of text/sound track or separate title |
eng |
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
Classification number |
1478,T |
100 ## - MAIN ENTRY--AUTHOR NAME |
Personal name |
Ishrat Younus |
110 ## - MAIN ENTRY--CORPORATE NAME |
Location of meeting |
Prof. Dr. Muhammad Ashraf |
245 ## - TITLE STATEMENT |
Title |
In Vitro Antiviral Activity Of Leaves Extracts Of Azadirachta Indica, Moringa Oleifera And Morus Alba Against Foot |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
Year of publication |
2012 |
502 ## - DISSERTATION NOTE |
Dissertation note |
The project was designed to assess in vitro antiviral and cytotoxic activity of leaves extracts of Azadirachta indica (AI), Moringa oleifera (MO) and Morus alba (MA) against Foot and Mouth disease virus (FMDV). Ethanolic, chloroformic and aqueous extracts of each plant were obtained by soxhlet apparatus. Chloroformic extracts were dissolved in cell culture media with the help of Dimethyl sulfoxide (DMSO). Eight concentrations 1 µg/ml, 6 µg/ml, 12 µg/ml, 25 µg/ml, 50 µg/ml, 100 µg/ml, 200 µg/ml and 400 µg/ml of each plant were used for both assays. Confluent BHK - 21 cells were grown in 96 well cell culture plates. Cells were treated by each concentration of extracts and extracts containing FMDV for cytotoxic and antiviral assay respectively in triplicate manner. Positive control (BHK-21 cells & cell culture media) and negative control (BHK-21 cells, FMDV & cell culture media) were kept for antiviral assay. For cytotoxic assay, positive and negative controls were kept as BHK-21 cells plus media and BHK-21 cells, media plus DMSO (20%) respectively. Cells viability and cytotoxic activity were determined by MTT assay for antiviral and cytotoxic assay respectively. Each extract was analyzed as cell survival percentage and expressed as means ± S.D. Statistical analysis was carried out by ANOVA. Seven plants extracts out of nine, exhibited antiviral activity against FMDV at a concentration non toxic to BHK-21 cell line. Ethanolic AI extract showed strongest anti-FMDV activity. Chloroformic MO leaves extracts showed significant antiviral activity. Chloroformic and aqueous MA leaves extract had no remarkable antiviral activity. At higher concentrations most of the plant extracts were cytotoxic
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650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical Term |
Department of Pharmaoclogy & Toxicology |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Dr. Aqeel Javeed |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Dr. Imran Altaf |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Koha item type |
Thesis |