000 -LEADER |
fixed length control field |
02062nam a2200181Ia 4500 |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20151006131202.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
150525s2012xx 000 0 und d |
041 ## - LANGUAGE CODE |
Language code of text/sound track or separate title |
eng |
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
Classification number |
1495,T |
100 ## - MAIN ENTRY--AUTHOR NAME |
Personal name |
Muhammad Asim Raza |
110 ## - MAIN ENTRY--CORPORATE NAME |
Location of meeting |
Dr. Arfan Ahmad |
245 ## - TITLE STATEMENT |
Title |
In Process Quality Control Factors Affecting The Potency Of Indigenous Mycoplasma Gallisepticum (Mg) |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
Year of publication |
2012 |
502 ## - DISSERTATION NOTE |
Dissertation note |
Mycoplasma gallisepticum (MG) is cause of chronic respiratory disease (CRD) and is responsible for significant economic losses to poultry industry. In Pakistan, imported MG bacterin fails to induce immunoprophylaxis that could be due to subtle antigenic variation in the immunogen of the vaccine. Therefore present study was conducted to optimize inactivations( phenol, formalin and binary ethylenimine) concentration and exposure time to inactivate MG and their effect on potency of MG bacterin ( prepared from local isolates) along with different bacterial biomasses (0.5%, 1% and 1.5% PCV) and adjuvants (montanide oil ,gel and water ). It was observed that the MG bacterin containing 1.5% level of immunogen/biomass induced significantly higher anti-MG-ELISA antibody titer (p < 0.05) as compared to other bacterins containing lower concentrations of the immunogen.The formaldehyde inactivated the pathogen within shortest possible time and showed undetectable effect on its potency. The antibody response was significantly higher (p<0.05) as compared to that of bacterins prepared from the pathogens inactivated by either phenol or BEI. . Montanide ISA70 containing MG bacterin induced significantly higher anti-MG-ELISA antibody titer (p<0.05) in broilers than the other bacterins containing either water or aluminum hydroxide gel. It is concluded that formaldehyde inactivated oil based vaccine containing one percent immunogen (0ne percent PCV) induce antibody response in broilers that is comparable with the imported vaccine.
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650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical Term |
Department of Microbiology |
700 ## - ADDED ENTRY--PERSONAL NAME |
Personal name |
Prof. Dr. Masood Rabbani |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Koha item type |
Thesis |