In Process Quality Control Factors Affecting The Potency Of Indigenous Mycoplasma Gallisepticum (Mg) (Record no. 3199)

000 -LEADER
fixed length control field 02062nam a2200181Ia 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20151006131202.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 150525s2012xx 000 0 und d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 1495,T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Muhammad Asim Raza
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Dr. Arfan Ahmad
245 ## - TITLE STATEMENT
Title In Process Quality Control Factors Affecting The Potency Of Indigenous Mycoplasma Gallisepticum (Mg)
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2012
502 ## - DISSERTATION NOTE
Dissertation note Mycoplasma gallisepticum (MG) is cause of chronic respiratory disease (CRD) and is responsible for significant economic losses to poultry industry. In Pakistan, imported MG bacterin fails to induce immunoprophylaxis that could be due to subtle antigenic variation in the immunogen of the vaccine. Therefore present study was conducted to optimize inactivations( phenol, formalin and binary ethylenimine) concentration and exposure time to inactivate MG and their effect on potency of MG bacterin ( prepared from local isolates) along with different bacterial biomasses (0.5%, 1% and 1.5% PCV) and adjuvants (montanide oil ,gel and water ). It was observed that the MG bacterin containing 1.5% level of immunogen/biomass induced significantly higher anti-MG-ELISA antibody titer (p < 0.05) as compared to other bacterins containing lower concentrations of the immunogen.The formaldehyde inactivated the pathogen within shortest possible time and showed undetectable effect on its potency. The antibody response was significantly higher (p<0.05) as compared to that of bacterins prepared from the pathogens inactivated by either phenol or BEI. . Montanide ISA70 containing MG bacterin induced significantly higher anti-MG-ELISA antibody titer (p<0.05) in broilers than the other bacterins containing either water or aluminum hydroxide gel. It is concluded that formaldehyde inactivated oil based vaccine containing one percent immunogen (0ne percent PCV) induce antibody response in broilers that is comparable with the imported vaccine.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Microbiology
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Prof. Dr. Masood Rabbani
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-05-29 1495,T 1495,T Thesis


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