Molecular Basis Of Antibiotic Resistanc In E. Coli Isolates From Poultry Drinking Water (Record no. 3201)
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|005 - DATE AND TIME OF LATEST TRANSACTION|
|008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION|
|fixed length control field||150525s2012xx 000 0 und d|
|041 ## - LANGUAGE CODE|
|Language code of text/sound track or separate title||eng|
|082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER|
|100 ## - MAIN ENTRY--AUTHOR NAME|
|Personal name||Hira Naseer|
|110 ## - MAIN ENTRY--CORPORATE NAME|
|Location of meeting||Ms. Sehrish Firyal|
|245 ## - TITLE STATEMENT|
|Title||Molecular Basis Of Antibiotic Resistanc In E. Coli Isolates From Poultry Drinking Water|
|260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)|
|Year of publication||2012|
|502 ## - DISSERTATION NOTE|
|Dissertation note||Back Ground:
E. coli is a single-celled organism belonging to the large bacterial family Enterobacteriaceae, the enteric bacteria. Most of the E. coli strains are harmless but there are some serotypes of it that are pathogenic and cause serious food poisoning in human and major economic losses in both chicken and turkeys. Poultry, the second largest industry in Pakistan is supplied by lots of antibiotics like streptomycin, sulfamethoxazole and tetracycline, streptomycin and ampicillin etc, either through feed or water. Use of antibiotics at large scale is resulting in the development of antibiotic resistance in poultry and human.
To check the molecular basis of antibiotic resistance in E.coli and to find out the genotypic and phenotypic correlation between resistant E.coli from poultry drinking water.
In this study, drinking water samples from poultry water were collected and cultured on MacConkey agar. Standard disk diffusion method will be used to check antibiotic susceptibility. Plasmid DNA was extracted from colonies showing antibiotic resistance by mini-prep protocol. Using universal set of primers, antibiotic resistant genes was amplified and then sequenced. The sequence thus obtained was compared in the database with previously reported sequences of antibiotic resistant gene in E. coli strains.
Prevalence of tetA and tetB genes was shown by a Column chart.
This study helped to find out prevalence of these antibiotic resistance gene in E. coli isolated from poultry drinking water, which are potential threats to human being.
|650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM|
|Topical Term||Institute of Biochemistry & Biotechnology|
|700 ## - ADDED ENTRY--PERSONAL NAME|
|Personal name||Prof. Dr. Masroor Ellahi Babar|
|942 ## - ADDED ENTRY ELEMENTS (KOHA)|
|Koha item type||Thesis|
|Damaged status||Collection code||Permanent Location||Current Location||Shelving location||Date acquired||Full call number||Accession Number||Koha item type|
|Veterinary Science||UVAS Library||UVAS Library||Thesis Section||2015-05-29||1497,T||1497,T||Thesis|