| 000 -LEADER |
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| fixed length control field |
02628nam a2200181Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20151006132805.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
150525s2012 xx 000 0 und d |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
1534,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
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| Personal name |
Haider Noor |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
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| Location of meeting |
Prof. Dr. Mansur-ud-Din Ahmad |
| 245 ## - TITLE STATEMENT |
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| Title |
Detection Of Carrier And Subclinical Infection Of Babesia Ovis Trough Pcr At Government Farms Of Punjab |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Year of publication |
2012 |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Haemoprotozoan infections in ruminants are of significant importance in tropical and subtropical regions of the world. Diseases are transmitted through ticks thus a number of epidemiological factors/risk factors are involved. Ovine Babesiosis is one such disease posing problems in Pakistan which is an agricultural country and livestock plays an important source of income for farmers. The economic losses in small ruminant production are significant in the tropical and subtropical regions of the world. Carrier sheep infected with Babesiosis are challenge to current diagnostic methods and are difficult to detect because of the low number of parasites in circulation. However diagnosis of carrier animals in herd is important for preventing outbreaks by transmission through vector ticks to healthy animals and for obtaining epidemiological data of disease. The work done on Babesia ovis is negligible. For this purpose a study was conducted at two farms to measure the prevalence and optimization of PCR for Babesia ovis. Blood was collected into an anticoagulant containing vacutainer. First thin smears were formed and stained with Giemsa stain for microscopic examination of Babesia ovis. For DNA extraction Puregene DNA purification system, Gentra, was used. Extracted DNA was amplified in a thermolyser using B. ovis primers and then analyzed using electrophoresis on 1% agarose gel. Microscopic examination demonstrated a prevalence of 16 % while PCR results revealed prevalence of 29% for B. ovis. Results displayed that the efficacy of PCR is more sensitive than Light Microscopy. Data on infection rate between male and female and between different age groups was statistically non-significant. Herd wise prevalence was 36% and 22% in Livestock Production Research Institute, Bahadarnagar, Okara and Small Ruminant Training and Research Center,Ravi Campus, Pattoki respectively. Common sites of attachment for the ticks were under the tail, perineal region and underneath ears.The data was analyzed by using SPSS (Statistical Package for Social Sciences).
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| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
Department of Epidemiology & Public Health |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dr. Muhammad Hassan Mushtaq |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Thesis |
