Bioconversion Of Whey To Beta-Galactosidase By Aspergillus Niger (Record no. 3304)
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000 -LEADER | |
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fixed length control field | 02475nam a2200193Ia 4500 |
005 - DATE AND TIME OF LATEST TRANSACTION | |
control field | 20151005151937.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 150525s2013 xx 000 0 und d |
041 ## - LANGUAGE CODE | |
Language code of text/sound track or separate title | eng |
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
Classification number | 1387,T |
100 ## - MAIN ENTRY--AUTHOR NAME | |
Personal name | Muhammad Tayyab Younas |
110 ## - MAIN ENTRY--CORPORATE NAME | |
Location of meeting | Ms. Huma Mujahid |
245 ## - TITLE STATEMENT | |
Title | Bioconversion Of Whey To Beta-Galactosidase By Aspergillus Niger |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
Year of publication | 2013 |
502 ## - DISSERTATION NOTE | |
Dissertation note | Beta-galactosidase (lactase) has catalytic property to hydrolyze lactose into glucose and galactose. It is extensively used for the synthesis of milk made products through fermentation. Food rich in lactose have variety of application in industrial and environmental processes. In present study production, purification andcharacterization of ?-galactosidase synthesized by Aspergillus niger has been considered as a great challenge. Beta-glactosidase is an important enzyme involved in conversion of lactose into glucose and galactose and produced on industrial scale for its large applications in the field of health, and food. The production of beta-galactosidase was carried out from fungal culture of Aspergillus niger using whey as a substrate. Optimization of different physical parameters such as temperature, pH, addition of corn steep liquor and production, purification and characterization of beta galactosidase enzyme from Aspergillus niger were studied. Optimum concentration of whey (4mL) were found 13.42 IU/mL and activity of beta galactosidase was found maximum at 72 h of incubation period and further incubation period decline the activity.Optimum pH (13.50 IU/mL)and temperature (17.67 IU/mL) were found 5.5 and 40°C respectively. Addition of corn steep liquor was enhanced the activity of beta galactosidase. Maximum activity was found with 0.6% of corn steep liquor which was 19.4IU/mLas compare to the other nitrogen sources. Finally, addition of ammonium sulphate ?-galactosidase was purified. ?-galactosidase was characterized considering ortho-Nitrophenyl-?-galactoside (ONPG) and whey as a substrate The purified beta-galactosidase was confirmed by SDS PAGE analysis which has molecular weight of 74kDa. The study could also establish that whey could effectively be utilized for ?- galactosidase production thus alleviating water pollution problems caused due to its disposal into the water streams. |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical Term | Institute of Biochemistry & Biotechnology |
700 ## - ADDED ENTRY--PERSONAL NAME | |
Personal name | Dr. Abu Saeed Hashmi |
700 ## - ADDED ENTRY--PERSONAL NAME | |
Personal name | Ms. Sehrish |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Koha item type | Thesis |
Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
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Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-05-29 | 1387,T | 1387,T | Thesis |