Dna Quantification Of Blood Stains Recovered From Washed Fabrics (Record no. 3371)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02446nam a2200193Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20151006141706.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150525s2013xx 000 0 und d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 1663,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Zeenat Salah-ud-din |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr. Muhammad Yasir Zahoor |
| 245 ## - TITLE STATEMENT | |
| Title | Dna Quantification Of Blood Stains Recovered From Washed Fabrics |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2013 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Bloodstains are extremely important forensic evidences for DNA profiling. Bloodstains recovered at crime scene can help to identify and confirm the credentials of the victim. Criminal can make all efforts to destroy and hide possible evidence at crime scene therefore these bloodstains on the crime place can hardly provide any evidence against the culprit. The criminal will try to take away or change clothes with the bloodstains of the victim. Once perpetrator has removed the bloodstains, culprit will feel secure and satisfied. But as a suspect if clothes are recovered with washed bloodstains and forensic evidence of DNA of the victim can be recovered from the washed clothes and can provide strong evidence against suspect. Present study has been planned to quantiate DNA from 10 different types of bloodstained fabrics with possible ways of washing to hide the blood from fabrics. 100 ìl of preservative blood was used in creating blood spot on fabric. After that dried bloodstained fabrics were hand washed with tap water and in detergent for 5 and 10mins and air dried at room temperature (25°C- 30°C) in laboratory. DNA was extracted through Chelex® 100 extraction procedure from these fabrics and analyzed on Real time PCR kit i.e. Quantifiler™ Human DNA Quantification kit which is specific for Human DNA. Research study showed there was no statistically significant difference in DNA concentrations among different washing methods (p-value = 0.318). There was statistically significant difference in DNA concentrations among different types of fabrics divided into two categories (p-value = 0.001, 0.002). Research findings showed that cellulose fibers recovered highest quantity of DNA, followed by artificial fibers and then protein fibers (cellulose >artificial>protein fiber) despite their washing methods. Thus provides a lead in solving such type of criminal cases. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Forensic Science |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Muhammad |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr. Muhammad Wasim |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-05-29 | 1663,T | 1663,T | Thesis |